Publication

Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins

Hjelm, A., Karyolaimos, A., Zhang, Z., Rujas, E., Vikström, D., Slotboom, D. J. & de Gier, J-W., Apr-2017, In : ACS Synthetic Biology. 6, 6, p. 985-994 10 p., acssynbio.6b00321.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Hjelm, A., Karyolaimos, A., Zhang, Z., Rujas, E., Vikström, D., Slotboom, D. J., & de Gier, J-W. (2017). Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins. ACS Synthetic Biology, 6(6), 985-994. [acssynbio.6b00321]. https://doi.org/10.1021/acssynbio.6b00321

Author

Hjelm, Anna ; Karyolaimos, Alexandros ; Zhang, Zhe ; Rujas, Edurne ; Vikström, David ; Slotboom, Dirk Jan ; de Gier, Jan-Willem. / Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins. In: ACS Synthetic Biology. 2017 ; Vol. 6, No. 6. pp. 985-994.

Harvard

Hjelm, A, Karyolaimos, A, Zhang, Z, Rujas, E, Vikström, D, Slotboom, DJ & de Gier, J-W 2017, 'Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins', ACS Synthetic Biology, vol. 6, no. 6, acssynbio.6b00321, pp. 985-994. https://doi.org/10.1021/acssynbio.6b00321

Standard

Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins. / Hjelm, Anna; Karyolaimos, Alexandros; Zhang, Zhe; Rujas, Edurne; Vikström, David; Slotboom, Dirk Jan; de Gier, Jan-Willem.

In: ACS Synthetic Biology, Vol. 6, No. 6, acssynbio.6b00321, 04.2017, p. 985-994.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Hjelm A, Karyolaimos A, Zhang Z, Rujas E, Vikström D, Slotboom DJ et al. Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins. ACS Synthetic Biology. 2017 Apr;6(6):985-994. acssynbio.6b00321. https://doi.org/10.1021/acssynbio.6b00321


BibTeX

@article{3767fa6ba11b4663b70f74b2abdd6392,
title = "Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins",
abstract = "Membrane and secretory protein production in Escherichia coli requires precisely controlled production rates to avoid the deleterious saturation of their biogenesis pathways. Based on this requirement, the E. coli L-rhamnose PBAD promoter (PrhaBAD) is often used for membrane and secretory protein production since PrhaBAD is thought to regulate protein production rates in an L-rhamnose concentration dependent manner. By monitoring protein production in real-time in E. coli wild-type and an L-rhamnose catabolism deficient mutant, we demonstrate that the L-rhamnose concentration-dependent tunability of PrhaBAD-mediated protein production is actually due to L-rhamnose consumption rather than regulating production rates. Using this information, a RhaT-mediated L-rhamnose transport and L-rhamnose catabolism deficient double mutant was constructed. We show that this mutant enables to regulate PrhaBAD-based protein production rates in an L-rhamnose concentration dependent manner and that this is critical to optimize membrane and secretory protein production yields. The high precision of protein production rates provided by the PrhaBAD promoter in an L-rhamnose transport and catabolism deficient background could also benefit other applications in synthetic biology.",
author = "Anna Hjelm and Alexandros Karyolaimos and Zhe Zhang and Edurne Rujas and David Vikstr{\"o}m and Slotboom, {Dirk Jan} and {de Gier}, Jan-Willem",
year = "2017",
month = "4",
doi = "10.1021/acssynbio.6b00321",
language = "English",
volume = "6",
pages = "985--994",
journal = "ACS Synthetic Biology",
issn = "2161-5063",
publisher = "AMER CHEMICAL SOC",
number = "6",

}

RIS

TY - JOUR

T1 - Tailoring Escherichia coli for the L-rhamnose PBAD promoter-based production of membrane and secretory proteins

AU - Hjelm, Anna

AU - Karyolaimos, Alexandros

AU - Zhang, Zhe

AU - Rujas, Edurne

AU - Vikström, David

AU - Slotboom, Dirk Jan

AU - de Gier, Jan-Willem

PY - 2017/4

Y1 - 2017/4

N2 - Membrane and secretory protein production in Escherichia coli requires precisely controlled production rates to avoid the deleterious saturation of their biogenesis pathways. Based on this requirement, the E. coli L-rhamnose PBAD promoter (PrhaBAD) is often used for membrane and secretory protein production since PrhaBAD is thought to regulate protein production rates in an L-rhamnose concentration dependent manner. By monitoring protein production in real-time in E. coli wild-type and an L-rhamnose catabolism deficient mutant, we demonstrate that the L-rhamnose concentration-dependent tunability of PrhaBAD-mediated protein production is actually due to L-rhamnose consumption rather than regulating production rates. Using this information, a RhaT-mediated L-rhamnose transport and L-rhamnose catabolism deficient double mutant was constructed. We show that this mutant enables to regulate PrhaBAD-based protein production rates in an L-rhamnose concentration dependent manner and that this is critical to optimize membrane and secretory protein production yields. The high precision of protein production rates provided by the PrhaBAD promoter in an L-rhamnose transport and catabolism deficient background could also benefit other applications in synthetic biology.

AB - Membrane and secretory protein production in Escherichia coli requires precisely controlled production rates to avoid the deleterious saturation of their biogenesis pathways. Based on this requirement, the E. coli L-rhamnose PBAD promoter (PrhaBAD) is often used for membrane and secretory protein production since PrhaBAD is thought to regulate protein production rates in an L-rhamnose concentration dependent manner. By monitoring protein production in real-time in E. coli wild-type and an L-rhamnose catabolism deficient mutant, we demonstrate that the L-rhamnose concentration-dependent tunability of PrhaBAD-mediated protein production is actually due to L-rhamnose consumption rather than regulating production rates. Using this information, a RhaT-mediated L-rhamnose transport and L-rhamnose catabolism deficient double mutant was constructed. We show that this mutant enables to regulate PrhaBAD-based protein production rates in an L-rhamnose concentration dependent manner and that this is critical to optimize membrane and secretory protein production yields. The high precision of protein production rates provided by the PrhaBAD promoter in an L-rhamnose transport and catabolism deficient background could also benefit other applications in synthetic biology.

U2 - 10.1021/acssynbio.6b00321

DO - 10.1021/acssynbio.6b00321

M3 - Article

VL - 6

SP - 985

EP - 994

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

SN - 2161-5063

IS - 6

M1 - acssynbio.6b00321

ER -

ID: 40010699