Synthesis of branched polyglucans by the tandem action of potato phosphorylase and Deinococcus geothermalis glycogen branching enzymevan der Vlist, J., Reixach, M. P., van der Maarel, M., Dijkhuizen, L., Schouten, A. J. & Loos, K., 1-Aug-2008, In : Macromolecular Rapid Communications. 29, 15, p. 1293-1297 5 p.
Research output: Contribution to journal › Article › Academic › peer-review
An enzymatic tandem reaction is described in which the enzymes phosphorylase and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) catalyze the synthesis of branched polyglucans from glucose-1-phosphate (G-1-P). Phosphorylase consumes G-1-P and polymerizes linear amylose while DR GBE introduces branching points on the alpha-(1 -> 6) positions by reshuffling short oligosaccharides. The resulting branched polyglucans have an unusually high degree of branching of 11%.
|Number of pages||5|
|Journal||Macromolecular Rapid Communications|
|Publication status||Published - 1-Aug-2008|
- enzymatic polymerization, glycogen branching enzyme, (1)H NMR, phosphorylase, polysaccharides, synthesis, GLUCAN PHOSPHORYLASE, BLOCK-COPOLYMERS, AMYLOSE, STARCH, POLYMERIZATION, POLYMERS