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Synthesis of branched polyglucans by the tandem action of potato phosphorylase and Deinococcus geothermalis glycogen branching enzyme

van der Vlist, J., Reixach, M. P., van der Maarel, M., Dijkhuizen, L., Schouten, A. J. & Loos, K., 1-Aug-2008, In : Macromolecular Rapid Communications. 29, 15, p. 1293-1297 5 p.

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DOI

An enzymatic tandem reaction is described in which the enzymes phosphorylase and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) catalyze the synthesis of branched polyglucans from glucose-1-phosphate (G-1-P). Phosphorylase consumes G-1-P and polymerizes linear amylose while DR GBE introduces branching points on the alpha-(1 -> 6) positions by reshuffling short oligosaccharides. The resulting branched polyglucans have an unusually high degree of branching of 11%.

Original languageEnglish
Pages (from-to)1293-1297
Number of pages5
JournalMacromolecular Rapid Communications
Volume29
Issue number15
Publication statusPublished - 1-Aug-2008

    Keywords

  • enzymatic polymerization, glycogen branching enzyme, (1)H NMR, phosphorylase, polysaccharides, synthesis, GLUCAN PHOSPHORYLASE, BLOCK-COPOLYMERS, AMYLOSE, STARCH, POLYMERIZATION, POLYMERS

ID: 4762572