Synthesis and processing of escherichia-coli tem-beta-lactamase and bacillus-licheniformis alpha-amylase in escherichia-coli: The role of signal peptidase-ivan Dijl, J. M., Smith, H., Bron, S. & Venema, G., Sep-1988, In : Molecular and General Genetics. 214, 1, p. 55-61 7 p.
Research output: Contribution to journal › Article › Academic › peer-review
A mutant of Escherichia coli, in which signal peptidase I synthesis can be regulated, was constructed. The mutant was used to study the effects of signal peptidase I limitation on the synthesis and efficiency of processing of two proteins: the periplasmic E. coli TEM-beta-lactamase and Bacillus licheniformis alpha-amylase, which also accumulates in the periplasm of E. coli. Signal peptidase I limitation resulted in reduced rates of processing of pre-beta-lactamase and in strong inhibition of synthesis of alpha-amylase. The data suggest that beta-lactamase is processed post-translationally and that an intimate relationship exists between the synthesis and processing of alpha-amylase.
|Number of pages||7|
|Journal||Molecular and General Genetics|
|Publication status||Published - Sep-1988|
- Bacillus, Bacterial Proteins, Biological Transport, Active, Cloning, Molecular, Endopeptidases, Escherichia coli, Gene Expression Regulation, Membrane Proteins, Mutation, Plasmids, Protein Processing, Post-Translational, Restriction Mapping, Serine Endopeptidases, alpha-Amylases, beta-Lactamases