Publication

Survival of genetically marked Escherichia coli O157: H7 in soil as affected by soil microbial community shifts

van Elsas, J. D., Hill, P., Chronakova, A., Grekova, M., Topalova, Y., Elhottova, D., Kristufek, V., Chroakova, A. & Kristfek, V., Jul-2007, In : ISME Journal. 1, 3, p. 204-214 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van Elsas, J. D., Hill, P., Chronakova, A., Grekova, M., Topalova, Y., Elhottova, D., ... Kristfek, V. (2007). Survival of genetically marked Escherichia coli O157: H7 in soil as affected by soil microbial community shifts. ISME Journal, 1(3), 204-214. https://doi.org/10.1038/ismej.2007.21

Author

van Elsas, Jan Dirk ; Hill, Patrick ; Chronakova, Alica ; Grekova, Martyna ; Topalova, Yana ; Elhottova, Dana ; Kristufek, Vaclav ; Chroakova, A. ; Kristfek, V. / Survival of genetically marked Escherichia coli O157 : H7 in soil as affected by soil microbial community shifts. In: ISME Journal. 2007 ; Vol. 1, No. 3. pp. 204-214.

Harvard

van Elsas, JD, Hill, P, Chronakova, A, Grekova, M, Topalova, Y, Elhottova, D, Kristufek, V, Chroakova, A & Kristfek, V 2007, 'Survival of genetically marked Escherichia coli O157: H7 in soil as affected by soil microbial community shifts', ISME Journal, vol. 1, no. 3, pp. 204-214. https://doi.org/10.1038/ismej.2007.21

Standard

Survival of genetically marked Escherichia coli O157 : H7 in soil as affected by soil microbial community shifts. / van Elsas, Jan Dirk; Hill, Patrick; Chronakova, Alica; Grekova, Martyna; Topalova, Yana; Elhottova, Dana; Kristufek, Vaclav; Chroakova, A.; Kristfek, V.

In: ISME Journal, Vol. 1, No. 3, 07.2007, p. 204-214.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van Elsas JD, Hill P, Chronakova A, Grekova M, Topalova Y, Elhottova D et al. Survival of genetically marked Escherichia coli O157: H7 in soil as affected by soil microbial community shifts. ISME Journal. 2007 Jul;1(3):204-214. https://doi.org/10.1038/ismej.2007.21


BibTeX

@article{5eebab0e972e4cef9a23790ccf4a239c,
title = "Survival of genetically marked Escherichia coli O157: H7 in soil as affected by soil microbial community shifts",
abstract = "A loamy sand soil sampled from a species- rich permanent grassland at a long- term experimental site ( Wildekamp, Bennekom, The Netherlands) was used to construct soil microcosms in which the microbial community compositions had been modified by fumigation at different intensities ( depths). As expected, increasing depth of fumigation was shown to result in progressively increasing effects on the microbiological soil parameters, as determined by cultivation- based as well as cultivation- independent ( PCR- DGGE, PLFA) methods. Both at 7 and at 60 days after fumigation, shifts in the bacterial, fungal and protozoan communities were noted, indicating that altered community compositions had emerged following a transition phase. At the level of bacteria culturable on plates, an increase of the prevalence of bacterial r- strategists was noted at 7 days followed by a decline at 60 days, which also hinted at the effectiveness of the fumigation treatments. The survival of a non- toxigenic Escherichia coli O157: H7 derivative, strain T, was then assessed over 60 days in these microcosms, using detection via colony forming units counts as well as via PCR- DGGE. Both data sets were consistent with each other. Thus, a clear effect of fumigation depth on the survival of the invading strain T was noted, as a progressive increase of depth coincided with a progressively enhanced inoculant survival rate. As fumigation depth was presumably inversely related to community complexity, this was consistent with the hypothesis that soil systems with reduced biological complexity offer enhanced opportunities for invading microbial species to establish and persist. The significance of this finding is discussed in the light of the ongoing discussion about the complexity - invasiveness relationship within microbial communities, in particular regarding the opportunities of pathogens to persist.",
keywords = "soil, fumigation, survival, Escherichia coli O157 : H7, bacterial community structure, molecular methods, AGRICULTURAL SOIL, BACTERIAL, DIVERSITY, FUMIGATION, MANURE, BIODIVERSITY, REGIMES, BIOMASS, GROWTH",
author = "{van Elsas}, {Jan Dirk} and Patrick Hill and Alica Chronakova and Martyna Grekova and Yana Topalova and Dana Elhottova and Vaclav Kristufek and A. Chroakova and V. Kristfek",
year = "2007",
month = "7",
doi = "10.1038/ismej.2007.21",
language = "English",
volume = "1",
pages = "204--214",
journal = "ISME Journal",
issn = "1751-7370",
publisher = "Nature Publishing Group",
number = "3",

}

RIS

TY - JOUR

T1 - Survival of genetically marked Escherichia coli O157

T2 - H7 in soil as affected by soil microbial community shifts

AU - van Elsas, Jan Dirk

AU - Hill, Patrick

AU - Chronakova, Alica

AU - Grekova, Martyna

AU - Topalova, Yana

AU - Elhottova, Dana

AU - Kristufek, Vaclav

AU - Chroakova, A.

AU - Kristfek, V.

PY - 2007/7

Y1 - 2007/7

N2 - A loamy sand soil sampled from a species- rich permanent grassland at a long- term experimental site ( Wildekamp, Bennekom, The Netherlands) was used to construct soil microcosms in which the microbial community compositions had been modified by fumigation at different intensities ( depths). As expected, increasing depth of fumigation was shown to result in progressively increasing effects on the microbiological soil parameters, as determined by cultivation- based as well as cultivation- independent ( PCR- DGGE, PLFA) methods. Both at 7 and at 60 days after fumigation, shifts in the bacterial, fungal and protozoan communities were noted, indicating that altered community compositions had emerged following a transition phase. At the level of bacteria culturable on plates, an increase of the prevalence of bacterial r- strategists was noted at 7 days followed by a decline at 60 days, which also hinted at the effectiveness of the fumigation treatments. The survival of a non- toxigenic Escherichia coli O157: H7 derivative, strain T, was then assessed over 60 days in these microcosms, using detection via colony forming units counts as well as via PCR- DGGE. Both data sets were consistent with each other. Thus, a clear effect of fumigation depth on the survival of the invading strain T was noted, as a progressive increase of depth coincided with a progressively enhanced inoculant survival rate. As fumigation depth was presumably inversely related to community complexity, this was consistent with the hypothesis that soil systems with reduced biological complexity offer enhanced opportunities for invading microbial species to establish and persist. The significance of this finding is discussed in the light of the ongoing discussion about the complexity - invasiveness relationship within microbial communities, in particular regarding the opportunities of pathogens to persist.

AB - A loamy sand soil sampled from a species- rich permanent grassland at a long- term experimental site ( Wildekamp, Bennekom, The Netherlands) was used to construct soil microcosms in which the microbial community compositions had been modified by fumigation at different intensities ( depths). As expected, increasing depth of fumigation was shown to result in progressively increasing effects on the microbiological soil parameters, as determined by cultivation- based as well as cultivation- independent ( PCR- DGGE, PLFA) methods. Both at 7 and at 60 days after fumigation, shifts in the bacterial, fungal and protozoan communities were noted, indicating that altered community compositions had emerged following a transition phase. At the level of bacteria culturable on plates, an increase of the prevalence of bacterial r- strategists was noted at 7 days followed by a decline at 60 days, which also hinted at the effectiveness of the fumigation treatments. The survival of a non- toxigenic Escherichia coli O157: H7 derivative, strain T, was then assessed over 60 days in these microcosms, using detection via colony forming units counts as well as via PCR- DGGE. Both data sets were consistent with each other. Thus, a clear effect of fumigation depth on the survival of the invading strain T was noted, as a progressive increase of depth coincided with a progressively enhanced inoculant survival rate. As fumigation depth was presumably inversely related to community complexity, this was consistent with the hypothesis that soil systems with reduced biological complexity offer enhanced opportunities for invading microbial species to establish and persist. The significance of this finding is discussed in the light of the ongoing discussion about the complexity - invasiveness relationship within microbial communities, in particular regarding the opportunities of pathogens to persist.

KW - soil

KW - fumigation

KW - survival

KW - Escherichia coli O157 : H7

KW - bacterial community structure

KW - molecular methods

KW - AGRICULTURAL SOIL

KW - BACTERIAL

KW - DIVERSITY

KW - FUMIGATION

KW - MANURE

KW - BIODIVERSITY

KW - REGIMES

KW - BIOMASS

KW - GROWTH

U2 - 10.1038/ismej.2007.21

DO - 10.1038/ismej.2007.21

M3 - Article

VL - 1

SP - 204

EP - 214

JO - ISME Journal

JF - ISME Journal

SN - 1751-7370

IS - 3

ER -

ID: 4605250