Publication

Surfactant treatment before reperfusion improves the immediate function of lung transplants in rats

Erasmus, ME., Petersen, AH., Hofstede, G., Haagsman, HP., Oetomo, SB. & Prop, J., Feb-1996, In : American Journal of Respiratory and Critical Care Medicine. 153, 2, p. 665-670 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

An impaired function of alveolar surfactant can cause lung transplant dysfunction early after reperfusion. In this study it was investigated whether treatment with surfactant before reperfusion improves the immediate function of lung transplants and whether an improved transplant function was associated with an increase in alveolar surfactant components. Left lungs with 6-h (n = 8) or prolonged 20-h ischemia (n = 10) were transplanted syngeneically in rats. In both ischemia groups half of the rung transplants were treated with surfactant just before reperfusion. Lung function was measured during reperfusion for 1 h. Thereafter, the rats were killed and bronchoalveolar ravage was performed to measure alveolar surfactant components. We found that surfactant treatment improved the immediate function of lung transplants in parallel with a higher amount of total surfactant phospholipids, a higher percentage of the heavy subtype of surfactant, a normalized percentage of phosphatidylcholine, and a higher amount of endogenous surfactant protein A (SP-A). We conclude that surfactant treatment before reperfusion does improve the immediate lung transplant function in rats in association with an increase in alveolar surfactant components. More particularly, the amount of (endogenous) SP-A is thought to be crucial for the efficacy of surfactant treatment after lung transplantation.

Original languageEnglish
Pages (from-to)665-670
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume153
Issue number2
Publication statusPublished - Feb-1996

    Keywords

  • LIPID EXTRACT SURFACTANT, PULMONARY SURFACTANT, PROTEIN-A, METABOLISM, RABBITS

View graph of relations

ID: 6455552