Publication

Suppression of the growth and export defects of an Escherichia coli secA(Ts) mutant by a gene cloned from Bacillus subtilis

Müller, J., Walter, F., van Dijl, J. M. & Behnke, D., Oct-1992, In : Molecular and General Genetics. 235, 1, p. 89-96 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

A gene library of Bacillus subtilis chromosomal DNA was screened for genes capable of reverting the growth defects of the Escherichia coli secA51(Ts) mutant at 42 degrees C. A B. subtilis gene, designated csaA, was found to phenotypically suppress not only the growth defects of the E. coli mutant, but also to relieve the detrimental accumulation of precursors of exported proteins. The csaA gene encoded a protein of 15 kDa (137 amino acids) and was likely to be the distalmost member of an operon. No similarity to csaA was found among DNA or protein sequences deposited in databases. In contrast to other homologous or heterologous suppressors of the E. coli secA51(Ts) mutation, the csaA gene did not exert pleiotropic effects on either the E. coli secY24(Ts) or lep9(Ts) mutations. However, it restored the ability of a SecB-deficient mutant to grow on complex medium. It is proposed that CsaA serves as a molecular chaperone for exported proteins or alternatively acts by stabilizing the SecA protein.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalMolecular and General Genetics
Volume235
Issue number1
Publication statusPublished - Oct-1992

    Keywords

  • PROTEIN SECRETION, SUPPRESSOR GENE, SECA (TS) MUTATION, ESCHERICHIA-COLI, BACILLUS-SUBTILIS, PROTEIN EXPORT, BETA-LACTAMASE, PRECURSOR PROTEINS, MEMBRANE-PROTEINS, SEC-Y, GROEL, TRANSLOCATION, SECRETION, CLONING, HOMOLOG

ID: 14879939