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Subunit a of the F1F0 ATP Synthase Requires YidC and SecYEG for Membrane Insertion

Kol, S., Majczak, W., Heerlien, R., van der Berg, J. P., Nouwen, N. & Driessen, A. J. M., 31-Jul-2009, In : Journal of Molecular Biology. 390, 5, p. 893-901 9 p.

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  • Subunit a of the F1F0 ATP Synthase

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DOI

The insertion of inner membrane proteins in Escherichia coli occurs almost exclusively via the SecYEG pathway, while some membrane proteins require the membrane protein insertase YidC. In vitro analysis demonstrates that subunit a of the F1F0 ATP synthase (F(0)a) is strictly dependent on Ffh, SecYEG and YidC for its membrane insertion but independent of the proton motive force. The insertion of the first transmembrane segment of F(0)a also depends on Ffh and SecYEG but not on YidC, whereas the insertion is strongly dependent on the proton motive force, unlike the full-length F(0)a protein. These data demonstrate an extensive role of YidC in the assembly of the F-0 sector of the F1F0 ATP synthase. (C) 2009 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)893-901
Number of pages9
JournalJournal of Molecular Biology
Volume390
Issue number5
Publication statusPublished - 31-Jul-2009

    Keywords

  • membrane insertion, F1F0 ATP synthase subunit a, YidC, SecYEG, proton motive force, RESPIRATORY-CHAIN COMPLEXES, ESCHERICHIA-COLI YIDC, INNER-MEMBRANE, MITOCHONDRIAL ATPASE, PROTEIN BIOGENESIS, FTSH PROTEASE, F-0 SECTOR, H+-ATPASE, TRANSLOCASE, OVERPRODUCTION

ID: 4930970