Subcutaneous immunotherapy with purified Der p1 and 2 suppresses type 2 immunity in a murine asthma modelHesse, L., van Ieperen, N., Habraken, C., Petersen, A. H., Korn, S., Smilda, T., Goedewaagen, B., Ruiters, M. H., van der Graaf, A. C. & Nawijn, M. C., Apr-2018, In : Allergy. 73, 4, p. 862-874 13 p.
Research output: Contribution to journal › Article › Academic › peer-review
BackgroundAllergen-specific immunotherapy can induce long-term suppression of allergic symptoms, reduce medication use, and prevent exacerbations of allergic rhinitis and asthma. Current treatment is based on crude allergen extracts, which contain immunostimulatory components such as -glucans, chitins, and endotoxin. Use of purified or recombinant allergens might therefore increase efficacy of treatment.
AimsHere, we test application of purified natural group 1 and 2 allergens from Dermatophagoides pteronyssinus (Der p) for subcutaneous immunotherapy (SCIT) treatment in a house dust mite (HDM)-driven mouse model of allergic asthma.
Materials and methodsHDM-sensitized mice received SCIT with crude HDM extract, a mixture of purified Der p1 and 2 (DerP1/2), or placebo. Upon challenges, we measured specific immunoglobulin responses, allergen-induced ear swelling response (ESR), airway hyperresponsiveness (AHR), and inflammation in bronchoalveolar lavage fluid (BAL) and lung tissue.
ResultsESR measurement shows suppression of early allergic response in HDM-SCIT- and DerP1/2-SCIT-treated mice. Both HDM-SCIT and DerP1/2-SCIT are able to suppress AHR and eosinophilic inflammation. In contrast, only DerP1/2-SCIT is able to significantly suppress type 2 cytokines in lung tissue and BAL fluid. Moreover, DerP1/2-SCIT treatment is uniquely able suppress CCL20 and showed a trend toward suppression of IL-33, CCL17 and eotaxin levels in lung tissue.
DiscussionTaken together, these data show that purified DerP1/2-SCIT is able to not only suppress AHR and inflammation, but also has superior activity toward suppression of Th2 cells and HDM-induced activation of lung structural cells including airway epithelium.
ConclusionsWe postulate that treatment with purified natural major allergens derived from HDM will likely increase clinical efficacy of SCIT.
|Number of pages||13|
|Early online date||10-Jan-2018|
|Publication status||Published - Apr-2018|
- Allergen-specific immunotherapy, allergic asthma, house dust mites, purified natural Der p1 and 2, tolerance induction, HOUSE-DUST MITE, ALLERGEN-SPECIFIC IMMUNOTHERAPY, DERMATOPHAGOIDES-PTERONYSSINUS, AIRWAY INFLAMMATION, RESPONSES, VACCINES, DER-P-1, FARINAE, GENES, CELLS