Publication

Studying mechanosensitive ion channels with an automated patch clamp

Barthmes, M., Jose, M. D. F., Birkner, J. P., Brüggemann, A., Wahl-Schott, C. & Kocer, A., Mar-2014, In : European Biophysics Journal. 43, 2-3, p. 97-104 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Barthmes, M., Jose, M. D. F., Birkner, J. P., Brüggemann, A., Wahl-Schott, C., & Kocer, A. (2014). Studying mechanosensitive ion channels with an automated patch clamp. European Biophysics Journal, 43(2-3), 97-104. https://doi.org/10.1007/s00249-014-0944-2

Author

Barthmes, Maria ; Jose, Mac Donald F ; Birkner, Jan Peter ; Brüggemann, Andrea ; Wahl-Schott, Christian ; Kocer, Armagan. / Studying mechanosensitive ion channels with an automated patch clamp. In: European Biophysics Journal. 2014 ; Vol. 43, No. 2-3. pp. 97-104.

Harvard

Barthmes, M, Jose, MDF, Birkner, JP, Brüggemann, A, Wahl-Schott, C & Kocer, A 2014, 'Studying mechanosensitive ion channels with an automated patch clamp', European Biophysics Journal, vol. 43, no. 2-3, pp. 97-104. https://doi.org/10.1007/s00249-014-0944-2

Standard

Studying mechanosensitive ion channels with an automated patch clamp. / Barthmes, Maria; Jose, Mac Donald F; Birkner, Jan Peter; Brüggemann, Andrea; Wahl-Schott, Christian; Kocer, Armagan.

In: European Biophysics Journal, Vol. 43, No. 2-3, 03.2014, p. 97-104.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Barthmes M, Jose MDF, Birkner JP, Brüggemann A, Wahl-Schott C, Kocer A. Studying mechanosensitive ion channels with an automated patch clamp. European Biophysics Journal. 2014 Mar;43(2-3):97-104. https://doi.org/10.1007/s00249-014-0944-2


BibTeX

@article{766d7b5258e548c4918789eaa53b363e,
title = "Studying mechanosensitive ion channels with an automated patch clamp",
abstract = "Patch clamp electrophysiology is the main technique to study mechanosensitive ion channels (MSCs), however, conventional patch clamping is laborious and success and output depends on the skills of the operator. Even though automated patch systems solve these problems for other ion channels, they could not be applied to MSCs. Here, we report on activation and single channel analysis of a bacterial mechanosensitive ion channel using an automated patch clamp system. With the automated system, we could patch not only giant unilamellar liposomes but also giant Escherichia coli (E. coli) spheroplasts. The tension sensitivity and channel kinetics data obtained in the automated system were in good agreement with that obtained from the conventional patch clamp. The findings will pave the way to high throughput fundamental and drug screening studies on mechanosensitive ion channels.",
keywords = "Patch clamp electrophysiology, Automated patch clamp, Mechanosensitive channel of large conductance, Spheroplast, Giant unilamellar vesicles, ESCHERICHIA-COLI, MEMBRANE PATCHES, CELLS, PHYSIOLOGY, RESOLUTION, PRESSURE, PROTEIN, MSCL",
author = "Maria Barthmes and Jose, {Mac Donald F} and Birkner, {Jan Peter} and Andrea Br{\"u}ggemann and Christian Wahl-Schott and Armagan Kocer",
year = "2014",
month = "3",
doi = "10.1007/s00249-014-0944-2",
language = "English",
volume = "43",
pages = "97--104",
journal = "European Biophysics Journal",
issn = "0175-7571",
publisher = "SPRINGER",
number = "2-3",

}

RIS

TY - JOUR

T1 - Studying mechanosensitive ion channels with an automated patch clamp

AU - Barthmes, Maria

AU - Jose, Mac Donald F

AU - Birkner, Jan Peter

AU - Brüggemann, Andrea

AU - Wahl-Schott, Christian

AU - Kocer, Armagan

PY - 2014/3

Y1 - 2014/3

N2 - Patch clamp electrophysiology is the main technique to study mechanosensitive ion channels (MSCs), however, conventional patch clamping is laborious and success and output depends on the skills of the operator. Even though automated patch systems solve these problems for other ion channels, they could not be applied to MSCs. Here, we report on activation and single channel analysis of a bacterial mechanosensitive ion channel using an automated patch clamp system. With the automated system, we could patch not only giant unilamellar liposomes but also giant Escherichia coli (E. coli) spheroplasts. The tension sensitivity and channel kinetics data obtained in the automated system were in good agreement with that obtained from the conventional patch clamp. The findings will pave the way to high throughput fundamental and drug screening studies on mechanosensitive ion channels.

AB - Patch clamp electrophysiology is the main technique to study mechanosensitive ion channels (MSCs), however, conventional patch clamping is laborious and success and output depends on the skills of the operator. Even though automated patch systems solve these problems for other ion channels, they could not be applied to MSCs. Here, we report on activation and single channel analysis of a bacterial mechanosensitive ion channel using an automated patch clamp system. With the automated system, we could patch not only giant unilamellar liposomes but also giant Escherichia coli (E. coli) spheroplasts. The tension sensitivity and channel kinetics data obtained in the automated system were in good agreement with that obtained from the conventional patch clamp. The findings will pave the way to high throughput fundamental and drug screening studies on mechanosensitive ion channels.

KW - Patch clamp electrophysiology

KW - Automated patch clamp

KW - Mechanosensitive channel of large conductance

KW - Spheroplast

KW - Giant unilamellar vesicles

KW - ESCHERICHIA-COLI

KW - MEMBRANE PATCHES

KW - CELLS

KW - PHYSIOLOGY

KW - RESOLUTION

KW - PRESSURE

KW - PROTEIN

KW - MSCL

U2 - 10.1007/s00249-014-0944-2

DO - 10.1007/s00249-014-0944-2

M3 - Article

C2 - 24526306

VL - 43

SP - 97

EP - 104

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - 2-3

ER -

ID: 10900790