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Studying mechanosensitive ion channels with an automated patch clamp

Barthmes, M., Jose, M. D. F., Birkner, J. P., Brüggemann, A., Wahl-Schott, C. & Kocer, A., Mar-2014, In : European Biophysics Journal. 43, 2-3, p. 97-104 8 p.

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  • Studying mechanosensitive ion channels with an automated patch clamp

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DOI

Patch clamp electrophysiology is the main technique to study mechanosensitive ion channels (MSCs), however, conventional patch clamping is laborious and success and output depends on the skills of the operator. Even though automated patch systems solve these problems for other ion channels, they could not be applied to MSCs. Here, we report on activation and single channel analysis of a bacterial mechanosensitive ion channel using an automated patch clamp system. With the automated system, we could patch not only giant unilamellar liposomes but also giant Escherichia coli (E. coli) spheroplasts. The tension sensitivity and channel kinetics data obtained in the automated system were in good agreement with that obtained from the conventional patch clamp. The findings will pave the way to high throughput fundamental and drug screening studies on mechanosensitive ion channels.
Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalEuropean Biophysics Journal
Volume43
Issue number2-3
Publication statusPublished - Mar-2014

    Keywords

  • Patch clamp electrophysiology, Automated patch clamp, Mechanosensitive channel of large conductance, Spheroplast, Giant unilamellar vesicles, ESCHERICHIA-COLI, MEMBRANE PATCHES, CELLS, PHYSIOLOGY, RESOLUTION, PRESSURE, PROTEIN, MSCL

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