Publication

Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes

Meng, X., Gangoiti, J., Bai, Y., Pijning, T., Van Leeuwen, S. S. & Dijkhuizen, L., Jul-2016, In : Cellular and molecular life sciences. 73, 14, p. 2681-2706 26 p.

Research output: Contribution to journalArticleAcademicpeer-review

Lactic acid bacteria (LAB) are known to produce large amounts of α-glucan exopolysaccharides. Family GH70 glucansucrase (GS) enzymes catalyze the synthesis of these α-glucans from sucrose. The elucidation of the crystal structures of representative GS enzymes has advanced our understanding of their reaction mechanism, especially structural features determining their linkage specificity. In addition, with the increase of genome sequencing, more and more GS enzymes are identified and characterized. Together, such knowledge may promote the synthesis of α-glucans with desired structures and properties from sucrose. In the meantime, two new GH70 subfamilies (GTFB- and GTFC-like) have been identified as 4,6-α-glucanotransferases (4,6-α-GTs) that represent novel evolutionary intermediates between the family GH13 and "classical GH70 enzymes". These enzymes are not active on sucrose; instead, they use (α1 → 4) glucans (i.e. malto-oligosaccharides and starch) as substrates to synthesize novel α-glucans by introducing linear chains of (α1 → 6) linkages. All these GH70 enzymes are very interesting biocatalysts and hold strong potential for applications in the food, medicine and cosmetic industries. In this review, we summarize the microbiological distribution and the structure-function relationships of family GH70 enzymes, introduce the two newly identified GH70 subfamilies, and discuss evolutionary relationships between family GH70 and GH13 enzymes.

Original languageEnglish
Pages (from-to)2681-2706
Number of pages26
JournalCellular and molecular life sciences
Volume73
Issue number14
Publication statusPublished - Jul-2016

    Keywords

  • GH70, GH13, Glucansucrase, a,6-alpha-Glucanotransferase, structure-function, evolution, LACTIC-ACID BACTERIA, LACTOBACILLUS-REUTERI 121, ALPHA-AMYLASE FAMILY, MESENTEROIDES NRRL B-1355, STREPTOCOCCUS-MUTANS GLUCOSYLTRANSFERASES, 2 CATALYTIC DOMAINS, LEUCONOSTOC-MESENTEROIDES, MOLECULAR CHARACTERIZATION, SEQUENCE-ANALYSIS, SUCROSE ANALOGS

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