Publication

Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160

Berezikov, E., Blinov, A. G., Scherbik, S., Cox, C. K. & Case, S. T., 26-Nov-1998, In : Gene. 223, 1-2, p. 347-354 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Berezikov, E., Blinov, A. G., Scherbik, S., Cox, C. K., & Case, S. T. (1998). Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160. Gene, 223(1-2), 347-354. https://doi.org/10.1016/S0378-1119(98)00165-6

Author

Berezikov, Eugene ; Blinov, Alexander G. ; Scherbik, Svetlana ; Cox, Carol K. ; Case, Steven T. / Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160. In: Gene. 1998 ; Vol. 223, No. 1-2. pp. 347-354.

Harvard

Berezikov, E, Blinov, AG, Scherbik, S, Cox, CK & Case, ST 1998, 'Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160', Gene, vol. 223, no. 1-2, pp. 347-354. https://doi.org/10.1016/S0378-1119(98)00165-6

Standard

Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160. / Berezikov, Eugene; Blinov, Alexander G.; Scherbik, Svetlana; Cox, Carol K.; Case, Steven T.

In: Gene, Vol. 223, No. 1-2, 26.11.1998, p. 347-354.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Berezikov E, Blinov AG, Scherbik S, Cox CK, Case ST. Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160. Gene. 1998 Nov 26;223(1-2):347-354. https://doi.org/10.1016/S0378-1119(98)00165-6


BibTeX

@article{817b1199d60b4495924c26094f1dd72e,
title = "Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160",
abstract = "cDNA encoding Chironomus thummi ssp160 was used to isolate a genomic clone that hybridized in situ to band A2b on polytene chromosome IV, the site of the ssp160 gene. DNA sequencing, primer extension and gene/cDNA nucleotide sequence alignment revealed the gene contains six exons and five introns; 70{\%} of ssp160 is encoded in exon 3. Variations between cDNA and gene sequences led to the design of a polymerase chain reaction, restriction fragment length polymorphism assay that was subsequently used to demonstrate the existence of polymorphic alleles whose distribution varied between geographically separated populations of larvae. The polymorphism is associated with codon deletions in a six-amino-acid repeat containing an N-linked glycosylation motif. These deletions may have resulted from slipped-strand mispairing during DNA replication.",
keywords = "Exons, In-situ hybridization, Introns, Nucleotide sequence variants, Repeated glycosylation motifs, Restriction fragment length polymorphism, Transcription start point",
author = "Eugene Berezikov and Blinov, {Alexander G.} and Svetlana Scherbik and Cox, {Carol K.} and Case, {Steven T.}",
year = "1998",
month = "11",
day = "26",
doi = "10.1016/S0378-1119(98)00165-6",
language = "English",
volume = "223",
pages = "347--354",
journal = "Gene",
issn = "0378-1119",
number = "1-2",

}

RIS

TY - JOUR

T1 - Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160

AU - Berezikov, Eugene

AU - Blinov, Alexander G.

AU - Scherbik, Svetlana

AU - Cox, Carol K.

AU - Case, Steven T.

PY - 1998/11/26

Y1 - 1998/11/26

N2 - cDNA encoding Chironomus thummi ssp160 was used to isolate a genomic clone that hybridized in situ to band A2b on polytene chromosome IV, the site of the ssp160 gene. DNA sequencing, primer extension and gene/cDNA nucleotide sequence alignment revealed the gene contains six exons and five introns; 70% of ssp160 is encoded in exon 3. Variations between cDNA and gene sequences led to the design of a polymerase chain reaction, restriction fragment length polymorphism assay that was subsequently used to demonstrate the existence of polymorphic alleles whose distribution varied between geographically separated populations of larvae. The polymorphism is associated with codon deletions in a six-amino-acid repeat containing an N-linked glycosylation motif. These deletions may have resulted from slipped-strand mispairing during DNA replication.

AB - cDNA encoding Chironomus thummi ssp160 was used to isolate a genomic clone that hybridized in situ to band A2b on polytene chromosome IV, the site of the ssp160 gene. DNA sequencing, primer extension and gene/cDNA nucleotide sequence alignment revealed the gene contains six exons and five introns; 70% of ssp160 is encoded in exon 3. Variations between cDNA and gene sequences led to the design of a polymerase chain reaction, restriction fragment length polymorphism assay that was subsequently used to demonstrate the existence of polymorphic alleles whose distribution varied between geographically separated populations of larvae. The polymorphism is associated with codon deletions in a six-amino-acid repeat containing an N-linked glycosylation motif. These deletions may have resulted from slipped-strand mispairing during DNA replication.

KW - Exons

KW - In-situ hybridization

KW - Introns

KW - Nucleotide sequence variants

KW - Repeated glycosylation motifs

KW - Restriction fragment length polymorphism

KW - Transcription start point

U2 - 10.1016/S0378-1119(98)00165-6

DO - 10.1016/S0378-1119(98)00165-6

M3 - Article

VL - 223

SP - 347

EP - 354

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -

ID: 75800717