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Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160

Berezikov, E., Blinov, A. G., Scherbik, S., Cox, C. K. & Case, S. T., 26-Nov-1998, In : Gene. 223, 1-2, p. 347-354 8 p.

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  • Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp1601

    Final publisher's version, 617 KB, PDF-document

DOI

cDNA encoding Chironomus thummi ssp160 was used to isolate a genomic clone that hybridized in situ to band A2b on polytene chromosome IV, the site of the ssp160 gene. DNA sequencing, primer extension and gene/cDNA nucleotide sequence alignment revealed the gene contains six exons and five introns; 70% of ssp160 is encoded in exon 3. Variations between cDNA and gene sequences led to the design of a polymerase chain reaction, restriction fragment length polymorphism assay that was subsequently used to demonstrate the existence of polymorphic alleles whose distribution varied between geographically separated populations of larvae. The polymorphism is associated with codon deletions in a six-amino-acid repeat containing an N-linked glycosylation motif. These deletions may have resulted from slipped-strand mispairing during DNA replication.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalGene
Volume223
Issue number1-2
Publication statusPublished - 26-Nov-1998

    Keywords

  • Exons, In-situ hybridization, Introns, Nucleotide sequence variants, Repeated glycosylation motifs, Restriction fragment length polymorphism, Transcription start point

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