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Stress exposure results in increased peroxisomal levels of yeast Pnc1 and Gpd1, which are imported via a piggy-backing mechanism

Kumar, S., Singh, R., Williams, C. P. & van der Klei, I. J., Jan-2016, In : Biochimica et Biophysica Acta-Molecular Cell Research. 1863, 1, p. 148-156 9 p.

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  • Stress exposure results in increased peroxisomal levels of yeast Pnc1 and Gpd1, which are imported via a piggy-backing mechanism

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DOI

Saccharomyces cerevisiae glycerol phosphate dehydrogenase 1 (Gpd1) and nicotinamidase (Pnc1) are two stress-induced enzymes. Both enzymes are predominantly localised to peroxisomes at normal growth conditions, but were reported to localise to the cytosol and nucleus upon exposure of cells to stress. Import of both proteins into peroxisomes depends on the peroxisomal targeting signal 2 (PTS2) receptor Pex7. Gpd1 contains a PTS2, however, Pnc1 lacks this sequence.

Here we show that Pnc1 physically interacts with Gpd1, which is required for piggy-back import of Pnc1 into peroxisomes. Quantitative fluorescence microscopy analyses revealed that the levels of both proteins increased in peroxisomes and in the cytosol upon exposure of cells to stress. However, upon exposure of cells to stress we also observed enhanced cytosolic levels of the control PTS2 protein thiolase, when produced under control of the GPD1 promoter. This suggests that these conditions cause a partial defect in PTS2 protein import, probably because the PTS2 import pathway is easily saturated. (C) 2015 The Authors. Published by Elsevier B.V.

Original languageEnglish
Pages (from-to)148-156
Number of pages9
JournalBiochimica et Biophysica Acta-Molecular Cell Research
Volume1863
Issue number1
Publication statusPublished - Jan-2016

    Keywords

  • Peroxisome, Matrix protein import, Piggy-back import, Glycerol phosphate dehydrogenase, Nicotinamidase, Saccharomyces cerevisiae, SACCHAROMYCES-CEREVISIAE, HANSENULA-POLYMORPHA, GLYCEROL-3-PHOSPHATE DEHYDROGENASE, GENE DISRUPTION, BUDDING YEAST, PATHWAY, PROTEIN, METHYLGLYOXAL, NICOTINAMIDE, RESISTANCE

ID: 25453132