Publication

Stiffness of natural extra-cellular vesicles is governed by membrane protein content

Sorkin, R., Huisjes, R. H., Vorselen, D., Ofir-Birin, Y., Roos, W. H., Regev-Rudzki, N., Schiffelers, R. M. & Wuite, G. J., 1-Jul-2017, In : European Biophysics Journal. 46, Supplement1, p. S128 1 p.

Research output: Contribution to journalMeeting AbstractAcademic

APA

Sorkin, R., Huisjes, R. H., Vorselen, D., Ofir-Birin, Y., Roos, W. H., Regev-Rudzki, N., ... Wuite, G. J. (2017). Stiffness of natural extra-cellular vesicles is governed by membrane protein content. European Biophysics Journal, 46(Supplement1), S128. https://doi.org/10.1007/s00249-017-1222-x

Author

Sorkin, R. ; Huisjes, R.H. ; Vorselen, D. ; Ofir-Birin, Y. ; Roos, W.H. ; Regev-Rudzki, N. ; Schiffelers, R.M. ; Wuite, G.J. / Stiffness of natural extra-cellular vesicles is governed by membrane protein content. In: European Biophysics Journal. 2017 ; Vol. 46, No. Supplement1. pp. S128.

Harvard

Sorkin, R, Huisjes, RH, Vorselen, D, Ofir-Birin, Y, Roos, WH, Regev-Rudzki, N, Schiffelers, RM & Wuite, GJ 2017, 'Stiffness of natural extra-cellular vesicles is governed by membrane protein content', European Biophysics Journal, vol. 46, no. Supplement1, pp. S128. https://doi.org/10.1007/s00249-017-1222-x

Standard

Stiffness of natural extra-cellular vesicles is governed by membrane protein content. / Sorkin, R.; Huisjes, R.H.; Vorselen, D.; Ofir-Birin, Y.; Roos, W.H.; Regev-Rudzki, N.; Schiffelers, R.M.; Wuite, G.J.

In: European Biophysics Journal, Vol. 46, No. Supplement1, 01.07.2017, p. S128.

Research output: Contribution to journalMeeting AbstractAcademic

Vancouver

Sorkin R, Huisjes RH, Vorselen D, Ofir-Birin Y, Roos WH, Regev-Rudzki N et al. Stiffness of natural extra-cellular vesicles is governed by membrane protein content. European Biophysics Journal. 2017 Jul 1;46(Supplement1):S128. https://doi.org/10.1007/s00249-017-1222-x


BibTeX

@article{937aeee6c18146dcbeba48072374ae67,
title = "Stiffness of natural extra-cellular vesicles is governed by membrane protein content",
abstract = "Extracellular vesicles (EVs) are important mediators of intercellular communication, being involved both in maintaining normal physiology as well as spreading of a wide range of diseases. In order to successfully deliver their cargo, EVs need to be taken up by the target cells. Several studies suggest that successful cellular uptake of nanoparticles is affected by their mechanical properties. We propose that mechanical properties of EVs are important with respect to their function. We study mechanics of vesicles from red blood cells (RBC), both healthy and malaria parasite infected. Moreover, we examine the effect of cell temperature treatment on the mechanical properties of the secreted vesicles. To do so we perform a detailed AFMforce spectroscopy study and analyze our results using a Helfrich-model based theoretical framework to estimate the bending modulus of different vesicle populations. By simultaneously performing a systematic analysis of EV protein and lipid composition, we find that bending modulus values are significantly decreased upon increase in EV membrane protein content. Our results can provide better understanding of EVs function and new insights into the vesiculation process in health and disease.",
keywords = "lipid, membrane protein, conceptual framework, erythrocyte, exosome, lipid composition, membrane, nonhuman, Plasmodium, population model, rigidity, spectroscopy, thermal exposure",
author = "R. Sorkin and R.H. Huisjes and D. Vorselen and Y. Ofir-Birin and W.H. Roos and N. Regev-Rudzki and R.M. Schiffelers and G.J. Wuite",
year = "2017",
month = "7",
day = "1",
doi = "10.1007/s00249-017-1222-x",
language = "English",
volume = "46",
pages = "S128",
journal = "European Biophysics Journal",
issn = "0175-7571",
publisher = "SPRINGER",
number = "Supplement1",

}

RIS

TY - JOUR

T1 - Stiffness of natural extra-cellular vesicles is governed by membrane protein content

AU - Sorkin, R.

AU - Huisjes, R.H.

AU - Vorselen, D.

AU - Ofir-Birin, Y.

AU - Roos, W.H.

AU - Regev-Rudzki, N.

AU - Schiffelers, R.M.

AU - Wuite, G.J.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Extracellular vesicles (EVs) are important mediators of intercellular communication, being involved both in maintaining normal physiology as well as spreading of a wide range of diseases. In order to successfully deliver their cargo, EVs need to be taken up by the target cells. Several studies suggest that successful cellular uptake of nanoparticles is affected by their mechanical properties. We propose that mechanical properties of EVs are important with respect to their function. We study mechanics of vesicles from red blood cells (RBC), both healthy and malaria parasite infected. Moreover, we examine the effect of cell temperature treatment on the mechanical properties of the secreted vesicles. To do so we perform a detailed AFMforce spectroscopy study and analyze our results using a Helfrich-model based theoretical framework to estimate the bending modulus of different vesicle populations. By simultaneously performing a systematic analysis of EV protein and lipid composition, we find that bending modulus values are significantly decreased upon increase in EV membrane protein content. Our results can provide better understanding of EVs function and new insights into the vesiculation process in health and disease.

AB - Extracellular vesicles (EVs) are important mediators of intercellular communication, being involved both in maintaining normal physiology as well as spreading of a wide range of diseases. In order to successfully deliver their cargo, EVs need to be taken up by the target cells. Several studies suggest that successful cellular uptake of nanoparticles is affected by their mechanical properties. We propose that mechanical properties of EVs are important with respect to their function. We study mechanics of vesicles from red blood cells (RBC), both healthy and malaria parasite infected. Moreover, we examine the effect of cell temperature treatment on the mechanical properties of the secreted vesicles. To do so we perform a detailed AFMforce spectroscopy study and analyze our results using a Helfrich-model based theoretical framework to estimate the bending modulus of different vesicle populations. By simultaneously performing a systematic analysis of EV protein and lipid composition, we find that bending modulus values are significantly decreased upon increase in EV membrane protein content. Our results can provide better understanding of EVs function and new insights into the vesiculation process in health and disease.

KW - lipid

KW - membrane protein

KW - conceptual framework

KW - erythrocyte

KW - exosome

KW - lipid composition

KW - membrane

KW - nonhuman

KW - Plasmodium

KW - population model

KW - rigidity

KW - spectroscopy

KW - thermal exposure

U2 - 10.1007/s00249-017-1222-x

DO - 10.1007/s00249-017-1222-x

M3 - Meeting Abstract

VL - 46

SP - S128

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - Supplement1

ER -

ID: 46990381