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Staphylococcus aureus from the German general population is highly diverse

Becker, K., Schaumburg, F., Fegeler, C., Friedrich, A. W., Kock, R. & Prevalence Multiresistant Microorg, Jan-2017, In : International journal of medical microbiology. 307, 1, p. 21-27 7 p.

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  • Staphylococcus aureus from the German general population is highly diverse

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DOI

  • Karsten Becker
  • Frieder Schaumburg
  • Christian Fegeler
  • Alexander W. Friedrich
  • Robin Kock
  • Prevalence Multiresistant Microorg

Objectives: This prospective cohort study evaluates colonization dynamics and molecular characteristics of methicillin-susceptible and - resistant Staphylococcus aureus (MSSA/MRSA) in a German general population.

Methods: Nasal swabs of 1878 non-hospitalized adults were screened for S. aureus. Participants were screened thrice in intervals of 6-8 months. Isolates were characterized by spa and agr typing, mecA and mecC possession, respectively, and PCRs targeting virulence factors.

Results: 40.9% of all participants carried S. aureus at least once while 0.7% of the participants carried MRSA (Mainly spa t011). MSSA isolates (n =1359) were associated with 331 different spa types; t084 (7.7%), t091 (6.1%) and t012 (71, 5.2%) were predominant. Of 206 participants carrying S. aureus at all three sampling time points, 14.1% carried the same spa type continuously; 5.3% carried different spa types with similar repeat patterns, but 80.6% carried S. aureus with unrelated spa types. MSSA isolates frequently harboured genes encoding enterotoxins (sec: 16.6%, seg: 63.1%, sei: 64.5%) and toxic shock syndrome toxin (tst: 17.5%), but rarely Panton-Valentine leukocidin (lukS-PV IlukF-PV: 0.2%).

Conclusions: MSSA colonizing human nares in the community are clonally highly diverse. Among those constantly carrying S. aureus, clonal lineages changed over time. The proportion of persistent S. aureus carriers was lower than reported elsewhere. (C) 2016 Elsevier GmbH. All rights reserved.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalInternational journal of medical microbiology
Volume307
Issue number1
Early online date30-Nov-2016
Publication statusPublished - Jan-2017

    Keywords

  • Staphylococcus aureus, MRSA, Epidemiology, Livestock, Colonization, CC398, NASAL CARRIAGE, EVOLUTIONARY DYNAMICS, CLONAL COMPLEX, SPA TYPES, INFECTIONS, STRAINS, PREVALENCE, COLONIZATION, BLOOD

ID: 38856093