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Specific inulin-type fructan fibers protect against autoimmune diabetes by modulating gut immunity, barrier function, and microbiota homeostasis

Chen, K., Chen, H., Faas, M. M., de Haan, B. J., Li, J., Xiao, P., Zhang, H., Diana, J., de Vos, P. & Sun, J., Aug-2017, In : Molecular Nutrition & Food Research. 61, 8, p. 1-11 11 p., 1601006.

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  • Specific inulin-type fructan fibers protect against autoimmune diabetes by modulating gut immunity, barrier function, and microbiota homeostasis

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Scope: Dietary fibers capable of modifying gut barrier and microbiota homeostasis affect the progression of type 1 diabetes (T1D). Here, we aim to compare modulatory effects of inulin-type fructans (ITFs), natural soluble dietary fibers with different degrees of fermentability from chicory root, on T1D development in nonobese diabetic mice.

Methods and results: Female nonobese diabetic mice were weaned to long-and short-chain ITFs [ITF(l) and ITF(s), 5%] supplemented diet up to 24 weeks. T1D incidence, pancreatic-gut immune responses, gut barrier function, and microbiota composition were analyzed. ITF(l) but not ITF(s) supplementation dampened the incidence of T1D. ITF(l) promoted modulatory T-cell responses, as evidenced by increased CD25(+)Foxp3(+)CD4(+) regulatory T cells, decreased IL17A(+)CD4(+) Th17 cells, and modulated cytokine production profile in the pancreas, spleen, and colon. Furthermore, ITF(l) suppressed NOD like receptor protein 3 caspase-1-p20-IL-1 beta inflammasome in the colon. Expression of barrier reinforcing tight junction proteins occludin and claudin-2, antimicrobial peptides beta-defensin-1, and cathelicidin-related antimicrobial peptide as well as short-chain fatty acid production were enhanced by ITF(l). Next-generation sequencing analysis revealed that ITF(l) enhanced Firmicutes/Bacteroidetes ratio to an antidiabetogenic balance and enriched modulatory Ruminococcaceae and Lactobacilli.

Conclusion: Our data demonstrate that ITF(l) but not ITF(s) delays the development of T1D via modulation of gut-pancreatic immunity, barrier function, and microbiota homeostasis.

Original languageEnglish
Article number1601006
Pages (from-to)1-11
Number of pages11
JournalMolecular Nutrition & Food Research
Volume61
Issue number8
Publication statusPublished - Aug-2017

    Keywords

  • Barrier integrity, Dietary fibers, Gastroentero pancreatic regulation, Gut microbiota homeostasis, Immunomodulation, Nutritional intervention, Pancreatic-gut immunity, Type 1 diabetes pathophysiology, INTESTINAL EPITHELIAL-CELLS, REGULATORY T-CELLS, NOD MICE, ANTIMICROBIAL PEPTIDE, TH17 CELLS, ACTIVATION, BACTERIA, DIET, PERMEABILITY, DISEASE

ID: 40046929