Publication

Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria

Tiwari, V., Matutes, Y. A., Gardiner, A. T., Jansen, T. L. C., Cogdell, R. J. & Ogilvie, J. P., 11-Oct-2018, In : Nature Communications. 9, 10 p., 4219.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Tiwari, V., Matutes, Y. A., Gardiner, A. T., Jansen, T. L. C., Cogdell, R. J., & Ogilvie, J. P. (2018). Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria. Nature Communications, 9, [4219]. https://doi.org/10.1038/s41467-018-06619-x

Author

Tiwari, Vivek ; Matutes, Yassel Acosta ; Gardiner, Alastair T. ; Jansen, Thomas L. C. ; Cogdell, Richard J. ; Ogilvie, Jennifer P. / Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria. In: Nature Communications. 2018 ; Vol. 9.

Harvard

Tiwari, V, Matutes, YA, Gardiner, AT, Jansen, TLC, Cogdell, RJ & Ogilvie, JP 2018, 'Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria', Nature Communications, vol. 9, 4219. https://doi.org/10.1038/s41467-018-06619-x

Standard

Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria. / Tiwari, Vivek; Matutes, Yassel Acosta; Gardiner, Alastair T.; Jansen, Thomas L. C.; Cogdell, Richard J.; Ogilvie, Jennifer P.

In: Nature Communications, Vol. 9, 4219, 11.10.2018.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Tiwari V, Matutes YA, Gardiner AT, Jansen TLC, Cogdell RJ, Ogilvie JP. Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria. Nature Communications. 2018 Oct 11;9. 4219. https://doi.org/10.1038/s41467-018-06619-x


BibTeX

@article{e4454d8d075c42c08b1e4515601bc2c5,
title = "Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria",
abstract = "Conventional implementations of two-dimensional electronic spectroscopy typically spatially average over similar to 10(10) chromophores spread over similar to 10(4) micron square area, limiting their ability to characterize spatially heterogeneous samples. Here we present a variation of two-dimensional electronic spectroscopy that is capable of mapping spatially varying differences in excitonic structure, with sensitivity orders of magnitude better than conventional spatially-averaged electronic spectroscopies. The approach performs fluorescence-detection-based fully collinear two-dimensional electronic spectroscopy in a microscope, combining femtosecond time-resolution, sub-micron spatial resolution, and the sensitivity of fluorescence detection. We demonstrate the approach on a mixture of photosynthetic bacteria that are known to exhibit variations in electronic structure with growth conditions. Spatial variations in the constitution of mixed bacterial colonies manifests as spatially varying peak intensities in the measured two-dimensional contour maps, which exhibit distinct diagonal and cross-peaks that reflect differences in the excitonic structure of the bacterial proteins.",
keywords = "LIGHT-HARVESTING COMPLEXES, ENERGY-TRANSFER, RHODOPSEUDOMONAS-PALUSTRIS, QUANTUM COHERENCE, RAMAN-SCATTERING, 2 LH2, MICROSCOPY, RELAXATION, DYNAMICS, RECOMBINATION",
author = "Vivek Tiwari and Matutes, {Yassel Acosta} and Gardiner, {Alastair T.} and Jansen, {Thomas L. C.} and Cogdell, {Richard J.} and Ogilvie, {Jennifer P.}",
year = "2018",
month = "10",
day = "11",
doi = "10.1038/s41467-018-06619-x",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Spatially-resolved fluorescence-detected two-dimensional electronic spectroscopy probes varying excitonic structure in photosynthetic bacteria

AU - Tiwari, Vivek

AU - Matutes, Yassel Acosta

AU - Gardiner, Alastair T.

AU - Jansen, Thomas L. C.

AU - Cogdell, Richard J.

AU - Ogilvie, Jennifer P.

PY - 2018/10/11

Y1 - 2018/10/11

N2 - Conventional implementations of two-dimensional electronic spectroscopy typically spatially average over similar to 10(10) chromophores spread over similar to 10(4) micron square area, limiting their ability to characterize spatially heterogeneous samples. Here we present a variation of two-dimensional electronic spectroscopy that is capable of mapping spatially varying differences in excitonic structure, with sensitivity orders of magnitude better than conventional spatially-averaged electronic spectroscopies. The approach performs fluorescence-detection-based fully collinear two-dimensional electronic spectroscopy in a microscope, combining femtosecond time-resolution, sub-micron spatial resolution, and the sensitivity of fluorescence detection. We demonstrate the approach on a mixture of photosynthetic bacteria that are known to exhibit variations in electronic structure with growth conditions. Spatial variations in the constitution of mixed bacterial colonies manifests as spatially varying peak intensities in the measured two-dimensional contour maps, which exhibit distinct diagonal and cross-peaks that reflect differences in the excitonic structure of the bacterial proteins.

AB - Conventional implementations of two-dimensional electronic spectroscopy typically spatially average over similar to 10(10) chromophores spread over similar to 10(4) micron square area, limiting their ability to characterize spatially heterogeneous samples. Here we present a variation of two-dimensional electronic spectroscopy that is capable of mapping spatially varying differences in excitonic structure, with sensitivity orders of magnitude better than conventional spatially-averaged electronic spectroscopies. The approach performs fluorescence-detection-based fully collinear two-dimensional electronic spectroscopy in a microscope, combining femtosecond time-resolution, sub-micron spatial resolution, and the sensitivity of fluorescence detection. We demonstrate the approach on a mixture of photosynthetic bacteria that are known to exhibit variations in electronic structure with growth conditions. Spatial variations in the constitution of mixed bacterial colonies manifests as spatially varying peak intensities in the measured two-dimensional contour maps, which exhibit distinct diagonal and cross-peaks that reflect differences in the excitonic structure of the bacterial proteins.

KW - LIGHT-HARVESTING COMPLEXES

KW - ENERGY-TRANSFER

KW - RHODOPSEUDOMONAS-PALUSTRIS

KW - QUANTUM COHERENCE

KW - RAMAN-SCATTERING

KW - 2 LH2

KW - MICROSCOPY

KW - RELAXATION

KW - DYNAMICS

KW - RECOMBINATION

U2 - 10.1038/s41467-018-06619-x

DO - 10.1038/s41467-018-06619-x

M3 - Article

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 4219

ER -

ID: 66646084