Publication

Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns

Ebbing, A., Vertesy, A., Betist, M. C., Spanjaard, B., Junker, J. P., Berezikov, E., van Oudenaarden, A. & Korswagen, H. C., 17-Dec-2018, In : Developmental Cell. 47, 6, p. 801-813 19 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Ebbing, A., Vertesy, A., Betist, M. C., Spanjaard, B., Junker, J. P., Berezikov, E., van Oudenaarden, A., & Korswagen, H. C. (2018). Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns. Developmental Cell, 47(6), 801-813. https://doi.org/10.1016/j.devcel.2018.10.016

Author

Ebbing, Annabel ; Vertesy, Abel ; Betist, Marco C. ; Spanjaard, Bastiaan ; Junker, Jan Philipp ; Berezikov, Eugene ; van Oudenaarden, Alexander ; Korswagen, Hendrik C. / Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns. In: Developmental Cell. 2018 ; Vol. 47, No. 6. pp. 801-813.

Harvard

Ebbing, A, Vertesy, A, Betist, MC, Spanjaard, B, Junker, JP, Berezikov, E, van Oudenaarden, A & Korswagen, HC 2018, 'Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns', Developmental Cell, vol. 47, no. 6, pp. 801-813. https://doi.org/10.1016/j.devcel.2018.10.016

Standard

Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns. / Ebbing, Annabel; Vertesy, Abel; Betist, Marco C.; Spanjaard, Bastiaan; Junker, Jan Philipp; Berezikov, Eugene; van Oudenaarden, Alexander; Korswagen, Hendrik C.

In: Developmental Cell, Vol. 47, No. 6, 17.12.2018, p. 801-813.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Ebbing A, Vertesy A, Betist MC, Spanjaard B, Junker JP, Berezikov E et al. Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns. Developmental Cell. 2018 Dec 17;47(6):801-813. https://doi.org/10.1016/j.devcel.2018.10.016


BibTeX

@article{ac004c122fc247be8a158c38badcb7e2,
title = "Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns",
abstract = "To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region-and tissue-specific genes. We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.",
keywords = "MALE MATING-BEHAVIOR, NEUROPEPTIDE GENE, READ ALIGNMENT, GERM-LINE, SPERM, NEMATODE, NEURONS, VESICLES, FAMILY",
author = "Annabel Ebbing and Abel Vertesy and Betist, {Marco C.} and Bastiaan Spanjaard and Junker, {Jan Philipp} and Eugene Berezikov and {van Oudenaarden}, Alexander and Korswagen, {Hendrik C.}",
year = "2018",
month = dec,
day = "17",
doi = "10.1016/j.devcel.2018.10.016",
language = "English",
volume = "47",
pages = "801--813",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "CELL PRESS",
number = "6",

}

RIS

TY - JOUR

T1 - Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns

AU - Ebbing, Annabel

AU - Vertesy, Abel

AU - Betist, Marco C.

AU - Spanjaard, Bastiaan

AU - Junker, Jan Philipp

AU - Berezikov, Eugene

AU - van Oudenaarden, Alexander

AU - Korswagen, Hendrik C.

PY - 2018/12/17

Y1 - 2018/12/17

N2 - To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region-and tissue-specific genes. We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.

AB - To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region-and tissue-specific genes. We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.

KW - MALE MATING-BEHAVIOR

KW - NEUROPEPTIDE GENE

KW - READ ALIGNMENT

KW - GERM-LINE

KW - SPERM

KW - NEMATODE

KW - NEURONS

KW - VESICLES

KW - FAMILY

U2 - 10.1016/j.devcel.2018.10.016

DO - 10.1016/j.devcel.2018.10.016

M3 - Article

VL - 47

SP - 801

EP - 813

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 6

ER -

ID: 72763981