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SP-A-enriched surfactant for treatment of rat lung transplants with SP-A deficiency after storage and reperfusion

Erasmus, ME., Hofstede, GJH., Petersen, AH., Batenburg, JJ., Haagsman, HP., Oetomo, SB. & Prop, J., 15-Feb-2002, In : Transplantation. 73, 3, p. 348-352 5 p., UNSP 0041-1337/02/7303-348/0.

Research output: Contribution to journalArticleAcademicpeer-review

Background. The function of pulmonary surfactant is affected by lung transplantation, contributing to impaired lung transplant function. A decreased amount of surfactant protein-A (SP-A) after reperfusion is believed to contribute to the impaired surfactant function. Surfactant treatment has been shown to improve lung transplant function, but the effect is variable. We investigated whether SP-A enrichment of surfactant improved the efficacy of surfactant treatment in lung transplantation.

Methods. Left and right lungs of Lewis rats, inflated with 50% O-2, were stored for 20 hr at 8degreesC. Surfactant in bronchoalveolar lavage fluid from right lungs was investigated after storage (n=6). Left lungs were transplanted into syngeneic recipients and treated with SP-A-deficient surfactant (n=6) or SP-A-enriched surfactant (n=6) just before reperfusion. Air was instilled into untreated lung transplants (n=6). Sham operated (n=4) and normal (n=8) animals served as controls. Lung function was measured during 1 hr of reperfusion; surfactant components in bronchoalveolar lavage fluid were measured after reperfusion.

Results. After storage the amount of SP-A decreased by 27%, whereas surfactant phospholipids changed minimally. After reperfusion a further decrease of SP-A was paralleled by profound changes in surfactant phospholipids. Lung transplant function, however, remained relatively good. After instillation of SP-A-enriched surfactant, PO2 values were reached that approximated sham control PO2 values, whereas after SP-A-deficient surfactant treatment, the PO2 values did not improve.

Conclusion. Enrichment of surfactant with SP-A for treatment of lung transplants improves the efficacy of surfactant treatment.

Original languageEnglish
Article numberUNSP 0041-1337/02/7303-348/0
Pages (from-to)348-352
Number of pages5
JournalTransplantation
Volume73
Issue number3
Publication statusPublished - 15-Feb-2002

    Keywords

  • EXOGENOUS SURFACTANT, PROTEIN-A, PRESERVATION, INJURY, MODEL, INHIBITION

ID: 3999115