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SOX10 is as specific as S100 protein in detecting metastases of melanoma in lymph nodes and is recommended for sentinel lymph node assessment

EORTC Melanoma Group, Szumera-Ciećkiewicz, A., Bosisio, F., Teterycz, P., Antoranz, A., Delogu, F., Koljenović, S., van de Wiel, B. A., Blokx, W., van Kempen, L. C., Rutkowski, P., Christopher van Akkooi, A., Cook, M. & Massi, D., Sep-2020, In : European Journal of Cancer. 137, p. 175-182 8 p.

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  • EJC-D-20-00524_R1 (2)

    Final author's version, 2.29 MB, PDF document

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  • SOX10 is as specific as S100 protein in detecting metastases of melanoma in lymph nodes and is recommended for sentinel lymph node assessment

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DOI

  • EORTC Melanoma Group
  • Anna Szumera-Ciećkiewicz
  • Francesca Bosisio
  • Paweł Teterycz
  • Asier Antoranz
  • Francesco Delogu
  • Senada Koljenović
  • Bart A van de Wiel
  • Willeke Blokx
  • Léon C van Kempen
  • Piotr Rutkowski
  • Alexander Christopher van Akkooi
  • Martin Cook
  • Daniela Massi

Background: Sentinel lymph node (SLN) biopsy remains crucial for melanoma staging. The European Organisation for Research and Treatment of Cancer Melanoma Group recommends performing immunohistochemical stainings for reproducible identification of melanoma metastases. S100 protein (pS100) is a commonly used melanocytic antigen because of its high sensitivity in spite of relatively low specificity. SRY-related HMG-box 10 protein (SOX10) is a transcription factor characterising neural crest-derived cells. It is uniformly expressed mostly in the nuclei of melanocytes, neural, and myoepithelial cells. Pathologists sometimes prefer SOX10 as a melanoma marker, but it has not yet been investigated on a large-scale to confirm that it is reliable and recommendable for routine SLN evaluation. Methods: Four hundred one treatment-naïve lymph node (LN) metastatic melanomas were included in high-density tissue microarrays and were assessed for the presence of SOX10 and pS100 by immunohistochemistry. The slides were digitalised, shared and evaluated by a panel of experienced melanoma pathologists. Results: The vast majority of melanomas were double-positive for pS100 and SOX10 (93.2%); a small percentage of the cases (3.9%) were double-negative melanomas. Discordance between the two markers was observed: 1.9% pS100(−)/SOX10(+) and 0.75% pS100(+)/SOX10(−). SOX10 was not expressed by immune cell types in the LN, resulting in a less controversial interpretation of the staining. Conclusions: SOX10 is as equally specific as pS100 for the detection of melanoma metastases in LNs. The interpretation of SOX10 staining is highly reproducible among different centres and different pathologists because of the absence of staining of immune cells.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalEuropean Journal of Cancer
Volume137
Early online date8-Aug-2020
Publication statusPublished - Sep-2020

    Keywords

  • Melanoma, Metastasis, SOX10, pS100, Sentinel lymph node, Lymph node, True prognostic value, MALIGNANT-MELANOMA, WAARDENBURG-SYNDROME, DIAGNOSIS, TUMORS, CELL, IMMUNOHISTOCHEMISTRY, EXPRESSION, MARKER, NEOPLASMS, FAMILY

ID: 131461278