Publication

Sorting signals and regulation of cognate basolateral trafficking in myelin biogenesis

Klunder, B., Baron, W., Schrage, C., de Jonge, J., de Vries, H. & Hoekstra, D., Apr-2008, In : Journal of Neuroscience Research. 86, 5, p. 1007-1016 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

A detailed understanding of trafficking pathways in mature oligodendrocytes is essential for addressing issues aimed at controlling (re)myelination by modulating myelin-directed transport. Previously, we have shown that viral marker proteins HA and VSV G, on reaching the apical and basolateral surfaces of polarized epithelial cells, respectively, are primarily transported to the plasma membrane and myelin sheet, respectively, in oligodendrocytes (OLGs). In the present study, we demonstrated that in OLGs basolateral sorting signals similar to those in epithelial cells may target proteins to the myelin sheet, emphasizing the basolateral- and apical-like nature of the myelin sheet and plasma membrane, respectively. Thus, substitution of essential amino acids reverses the direction of targeting of these proteins, whereas elimination of apical targeting of HA coincides with its dissipation from detergent-resistant microdomains. Furthermore, protein kinase C activation negatively regulated transport of the OLG resident transmembrane protein PLP to the myelin sheet, like that of VSV G as shown previously, but did not affect the localization of the membrane-associated myelin-specific proteins MBP and CNP These data imply that several distinctly regulated pathways operate in myelin sheet directed-transport that at least partly rely on a cognate basolateral sorting signal. (C) 2007 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)1007-1016
Number of pages10
JournalJournal of Neuroscience Research
Volume86
Issue number5
Publication statusPublished - Apr-2008

    Keywords

  • PLP, oligodendrocyte, sorting signal, basolateral traffic, myelin, PROTEIN-KINASE-C, INFLUENZA-VIRUS HEMAGGLUTININ, CENTRAL-NERVOUS-SYSTEM, AMINO-ACID CHANGE, CYTOPLASMIC DOMAIN, MDCK CELLS, EPITHELIAL-CELLS, OLIGODENDROCYTE DIFFERENTIATION, PLASMA-MEMBRANE, POLARIZED CELLS

ID: 4706627