Publication

Soil bacteria show different tolerance ranges to an unprecedented disturbance

Nunes, I., Jurburg, S., Jacquiod, S., Brejnrod, A., Salles, J. F., Prieme, A. & Sorensen, S. J., Feb-2018, In : Biology and Fertility of Soils. 54, 2, p. 189-202 14 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • Soil bacteria show different tolerance ranges

    Final publisher's version, 1.88 MB, PDF document

    Request copy

DOI

  • Ines Nunes
  • Stephanie Jurburg
  • Samuel Jacquiod
  • Asker Brejnrod
  • Joana Falcao Salles
  • Anders Prieme
  • Soren J. Sorensen

Soil microbial communities have remarkable capacities to cope with ceaseless environmental changes, but little is known about their adaptation potential when facing an unprecedented disturbance. We tested the effect of incremental dose of microwaving on soil bacteria as a model of unprecedented stress. 16S rRNA gene qPCR at both the DNA and cDNA levels was used to characterize the total (DNA) and transcriptionally active (cDNA) fractions of the bacterial community. Amplicon sequencing of 16S rRNA gene transcripts was performed to decipher tolerance ranges within the community using the concept of functional response groups (FRGs). Increasing microwaving doses resulted in 90% loss in total and transcriptionally active bacterial communities after 6.8 and 4.7 min, respectively. Four distinct FRGs with peculiar phylogenetic signatures were identified, revealing a link between taxonomy and increasing stress doses. FRG1, the most sensitive group, was dominated by Actinobacteria. FRG2 and FRG3, with intermediate tolerance, displayed prevalence of Proteobacteria, while FRG4, the most resistant group, was driven by Firmicutes. While the most sensitive FRGs showed predictable responses linked to changes in temperature and soil water content associated with microwaving, more tolerant FRG4 members exhibited a stochastic response nested within the Firmicutes phylum, potentially revealing bet-hedging strategists. The concept of FRGs based on 16S rRNA gene transcripts stood as an efficient tool for unraveling bacterial survival strategies and tolerance ranges triggered by incremental doses of an unprecedented stress, with regard to phylogeny linkages.

Original languageEnglish
Pages (from-to)189-202
Number of pages14
JournalBiology and Fertility of Soils
Volume54
Issue number2
Publication statusPublished - Feb-2018

    Keywords

  • Soil bacteria, Biodiversity, Disturbance, RNA, Functional response group, Bet-hedging, SP-NOV, RIBOSOMAL-RNA, MICROBIAL COMMUNITIES, MICROWAVE-RADIATION, GEN. NOV., PARCHMENT DOCUMENTS, HOLY-GRAIL, R-PACKAGE, DIVERSITY, DNA

ID: 54022890