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Sodium thiosulfate ameliorates oxidative stress and preserves renal function in hyperoxaluric rats

Bijarnia, R. K., Bachtler, M., Chandak, P. G., van Goor, H. & Pasch, A., 30-Apr-2015, In : PLoS ONE. 10, 4, 16 p., e0124881.

Research output: Contribution to journalArticleAcademicpeer-review

  • Rakesh K. Bijarnia
  • Matthias Bachtler
  • Prakash G. Chandak
  • Harry van Goor
  • Andreas Pasch

BACKGROUND: Hyperoxaluria causes crystal deposition in the kidney, which leads to oxidative stress and to injury and damage of the renal epithelium. Sodium thiosulfate (STS, Na2S2O3) is an anti-oxidant, which has been used in human medicine for decades. The effect of STS on hyperoxaluria-induced renal damage is not known.

METHODS: Hyperoxaluria and renal injury were induced in healthy male Wistar rats by chronic exposure to ethylene glycol (EG, 0.75%) in the drinking water for 4 weeks. The treatment effects of STS, NaCl or Na2SO4 were compared. Furthermore, the effects of STS on oxalate-induced oxidative stress were investigated in vitro in renal LLC-PK1 cells.

RESULTS: Chronic EG exposure led to hyperoxaluria, oxidative stress, calcium oxalate crystalluria and crystal deposition in the kidneys. Whereas all tested compounds significantly reduced crystal load, only STS-treatment maintained tissue superoxide dismutase activity and urine 8-isoprostaglandin levels in vivo and preserved renal function. In in vitro studies, STS showed the ability to scavenge oxalate-induced ROS accumulation dose dependently, reduced cell-released hydrogen peroxide and preserved superoxide dismutase activity. As a mechanism explaining this finding, STS was able to directly inactivate hydrogen peroxide in cell-free experiments.

CONCLUSIONS: STS is an antioxidant, which preserves renal function in a chronic EG rat model. Its therapeutic use in oxidative-stress induced renal-failure should be considered.

Original languageEnglish
Article numbere0124881
Number of pages16
JournalPLoS ONE
Volume10
Issue number4
Publication statusPublished - 30-Apr-2015

    Keywords

  • OXALATE CRYSTAL DEPOSITION, CALCIUM-OXALATE, VASCULAR CALCIFICATION, HEMODIALYSIS-PATIENTS, STONE FORMATION, CELL INJURY, VITAMIN-E, KIDNEY, CALCIPHYLAXIS, DISEASE

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