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Smart probe for simultaneous detection of copper ion, pyrophosphate, and alkaline phosphatase in vitro and in clinical samples

Kiran, S., Khatik, R. & Schirhagl, R., Sep-2019, In : Analytical and Bioanalytical Chemistry. 411, 24, p. 6475-6485 11 p.

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  • Smart probe for simultaneous detection of copper ion, pyrophosphate, and alkaline phosphatase in vitro and in clinical samples

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DOI

Wilson's disease (WD), which might lead to acute liver failure, is an inherited disorder characterized by accumulation of copper (Cu2+) in the brain, the liver, and other vital organs. In the clinic, decreased serum alkaline phosphatase (ALP) concentration is used for WD diagnosis. But to the best of our knowledge, using a fluorescent probe to simultaneously detect multiple factors in WD (e.g., Cu2+, pyrophosphate (PPi), and ALP) has not been reported. Herein, we rationally designed a fluorescent switch (E)-8-((4-methylbenzylidene)amino)napthalen-1-amine (L) and successfully applied it for sequential and selective detections of Cu2+, PPi, and ALP in vitro, in living cells and synovial fluid samples with "Off," "On," and "Off" fluorescence signals, respectively. Considering the obvious correlations among Cu2+, PPi, and ALP in WD, we envision that our fluorescent probe L could be applied to in vitro diagnosing WD in the near future.

Original languageEnglish
Pages (from-to)6475-6485
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number24
Publication statusPublished - Sep-2019

    Keywords

  • Wilson's disease, Copper ion, Pyrophosphate, Alkaline phosphatase, Fluorescent probe, SELECTIVE FLUORESCENT CHEMOSENSOR, DOPED CARBON DOTS, ON-OFF ASSAY, SENSITIVE DETECTION, AQUEOUS-MEDIUM, SENSOR, CU2+, RECOGNITION, SWITCH, MECHANISMS

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