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Small Functional Peptides and Their Application in Superfunctionalizing Proteins

Billerbeck, S., Mar-2018, Synthetic Biology: Parts, Devices and Applications. Smolke, C., Lee, S. Y., Nielsen, J. & Stephanopoulos, G. (eds.). Wiley Online Library, Vol. 8. p. 217-235 19 p.

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  • Small Functional Peptides and Their Application in Superfunctionalizing Proteins

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This chapter gives a notion on the diversity of pharmaceutically relevant functions that can be adopted by peptides and discusses the potential impact that an extended knowledge about permissive sites could make to the field of therapeutic peptides. Identification of permissive sites within the mentioned proteins, all of which are spatially rather complex assemblies, indicates that other less challenging proteins might be able to accept even larger insertions at certain positions. The exploitation of the full potential of functional peptides for the engineering of synthetic chimeras seems to be limited by the available knowledge on permissive sites and the need for relatively labor‐intensive methods to identify them in a scaffold of interest. Therefore more comprehensive rational methods would be desirable that, together with recent advances in DNA modification methods on chromosome‐level, might be a step toward the exploitation of the full potential of superfunctionalized proteins.
Original languageEnglish
Title of host publicationSynthetic Biology
Subtitle of host publicationParts, Devices and Applications
EditorsChristina Smolke, Sang Yup Lee, Jens Nielsen, Gregory Stephanopoulos
PublisherWiley Online Library
Chapter11
Pages217-235
Number of pages19
Volume8
ISBN (Electronic)978-3-527-68809-8
ISBN (Print)978-3-527-33075-1
Publication statusPublished - Mar-2018
Externally publishedYes

    Keywords

  • synthetic biology, DNA modification methods, labor‐intensive methods, permissive sites, small functional peptides, superfunctionalized proteins, synthetic chimeras, therapeutic peptides

ID: 86481274