Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore

Stoddart, D., Heron, AJ., Mikhailova, E., Maglia, G. & Bayley, H., 12-May-2009, In : Proceedings of the National Academy of Science of the United States of America. 106, 19, p. 7702-7707 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

The sequencing of individual DNA strands with nanopores is under investigation as a rapid, low-cost platform in which bases are identified in order as the DNA strand is transported through a pore under an electrical potential. Although the preparation of solid-state nanopores is improving, biological nanopores, such as alpha-hemolysin (alpha HL), are advantageous because they can be precisely manipulated by genetic modification. Here, we show that the transmembrane beta-barrel of an engineered alpha HL pore contains 3 recognition sites that can be used to identify all 4 DNA bases in an immobilized single-stranded DNA molecule, whether they are located in an otherwise homopolymeric DNA strand or in a heteropolymeric strand. The additional steps required to enable nanopore DNA sequencing are outlined.
Original languageEnglish
Pages (from-to)7702-7707
Number of pages6
JournalProceedings of the National Academy of Science of the United States of America
Issue number19
Publication statusPublished - 12-May-2009
Externally publishedYes


  • alpha-hemolysin, DNA sequencing, genomics, protein engineering, protein pore

ID: 36008749