Publication

Single-Molecule Redox Blinking of Perylene Diimide Derivatives in Water

Cordes, T., Vogelsang, J., Anaya, M., Spagnuolo, C., Gietl, A., Summerer, W., Herrmann, A., Muellen, K., Tinnefeld, P. & Müllen, K., 24-Feb-2010, In : Journal of the American Chemical Society. 132, 7, p. 2404-2409 6 p.

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DOI

Dynamic developments in ultrasensitive and superresolution fluorescence microscopy call for improved fluorescence markers with increased photostability and new functionalities We used single-molecule spectroscopy to study water-soluble perylene dicarboximide fluorophores (PDI), which were immobilized in aqueous buffer by attaching the fluorophore to DNA Under these conditions bright fluorescence, comparable to that of single-molecule compatible organic fluorophores, is observed with homogeneous spectral and fluorescence decay time distributions. We additionally show how the fluorescence of the PDI can be controlled through photoinduced electron-transfer reactions by using different concentrations of reductants and oxidants, yielding either blinking or stable emission. We explain these properties by the redox potentials of PDI and the recently introduced ROXS (reducing and oxidizing system) concept Finally, we evaluate how this fluorescence control of PDIs can be used for superresolution "Blink-Microscopy" in aqueous or organic media and more generally for single-molecule spectroscopy

Original languageEnglish
Pages (from-to)2404-2409
Number of pages6
JournalJournal of the American Chemical Society
Volume132
Issue number7
Publication statusPublished - 24-Feb-2010

    Keywords

  • SUPERRESOLUTION MICROSCOPY, FLUORESCENCE SPECTROSCOPY, PHOTOPHYSICAL DYNAMICS, DYES, NANOSCOPY, BIOLOGY, FLUOROPHORES, CHROMOPHORES, DENDRIMER, PATHWAYS

ID: 5036207