Publication

Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures

Sousa, C., Golebiewska, A., Poovathingal, S. K., Kaoma, T., Pires-Afonso, Y., Martina, S., Coowar, D., Azuaje, F., Skupin, A., Balling, R., Biber, K., Niclou, S. P. & Michelucci, A., 1-Nov-2018, In : Embo Reports. 19, 11, 17 p., 46171.

Research output: Contribution to journalArticleAcademicpeer-review

  • Carole Sousa
  • Anna Golebiewska
  • Suresh K. Poovathingal
  • Tony Kaoma
  • Yolanda Pires-Afonso
  • Silvia Martina
  • Djalil Coowar
  • Francisco Azuaje
  • Alexander Skupin
  • Rudi Balling
  • Knut Biber
  • Simone P. Niclou
  • Alessandro Michelucci

Microglia are specialized parenchymal-resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single-cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)-injected mice. By excluding the contribution of other immune CNS-resident and peripheral cells, we show that microglia isolated from LPS-injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease-associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases.

Original languageEnglish
Article number46171
Number of pages17
JournalEmbo Reports
Volume19
Issue number11
Publication statusPublished - 1-Nov-2018

    Keywords

  • heterogeneity, lipopolysaccharide, microglia, neuroinflammation, single-cell RNA-seq, CENTRAL-NERVOUS-SYSTEM, LARGE GENE LISTS, FACTOR-BETA-S, RNA-SEQ, ALZHEIMERS-DISEASE, MESSENGER-RNA, NEURODEGENERATIVE DISEASE, MOUSE MODEL, RAT-BRAIN, EXPRESSION

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