Publication

Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells

Maimets, M., Bron, R., de Haan, G., van Os, R. & Coppes, R. P., Sep-2015, In : Radiotherapy and Oncology. 116, 3, p. 443-448 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Maimets, M., Bron, R., de Haan, G., van Os, R., & Coppes, R. P. (2015). Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells. Radiotherapy and Oncology, 116(3), 443-448. https://doi.org/10.1016/j.radonc.2015.06.022

Author

Maimets, Martti ; Bron, Reinier ; de Haan, Gerald ; van Os, Ronald ; Coppes, Robert P. / Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells. In: Radiotherapy and Oncology. 2015 ; Vol. 116, No. 3. pp. 443-448.

Harvard

Maimets, M, Bron, R, de Haan, G, van Os, R & Coppes, RP 2015, 'Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells', Radiotherapy and Oncology, vol. 116, no. 3, pp. 443-448. https://doi.org/10.1016/j.radonc.2015.06.022

Standard

Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells. / Maimets, Martti; Bron, Reinier; de Haan, Gerald; van Os, Ronald; Coppes, Robert P.

In: Radiotherapy and Oncology, Vol. 116, No. 3, 09.2015, p. 443-448.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Maimets M, Bron R, de Haan G, van Os R, Coppes RP. Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells. Radiotherapy and Oncology. 2015 Sep;116(3):443-448. https://doi.org/10.1016/j.radonc.2015.06.022


BibTeX

@article{03fa9277aba0435ea4e03f191ef0dd2c,
title = "Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells",
abstract = "Background and purpose: Salivary gland dysfunction is a major side effect of radiotherapy for head and neck cancer patients, which in the future might be salvaged by autologous adult salivary gland stem cell (SGSC) therapy. Since frail elderly patients may have decreased activity of SGSCs, we aimed to characterize the potential of aged SGSC-population in a murine model.Materials and methods: Salivary glands and salisphere-derived cells from young and old mice were tested for CD24 and CD29 stem cell marker expression using FACS. Moreover, in vitro expansion capability and in vivo regeneration potential upon post-irradiation transplantation of young and aged SGSCs were measured.Results: An increase in CD24(hi)/CD29(hi) putative stem cells was detected in aged salivary glands albeit with a decrease in functional ability to form salispheres. However, the salispheres formed from aged mice salivary glands expressed CD24(hi)/CD29(hi) to the same extent as the ones from young mice. Moreover, following exposure to adequate growth conditions old and young SGSCs exhibited similar in vitro expansion- and in vivo regeneration potential.Conclusions: Aged SGSCs although reduced in number are in vitro indistinguishable from young SGSCs and could potentially be used to ameliorate age- or treatment related salivary gland dysfunction. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
keywords = "Radiation-xerostomia, Salivary glands, Stem cells, Regeneration, Aging, MUSCLE, TRANSPLANTATION",
author = "Martti Maimets and Reinier Bron and {de Haan}, Gerald and {van Os}, Ronald and Coppes, {Robert P.}",
note = "Copyright {\circledC} 2015. Published by Elsevier Ireland Ltd.",
year = "2015",
month = "9",
doi = "10.1016/j.radonc.2015.06.022",
language = "English",
volume = "116",
pages = "443--448",
journal = "Radiotherapy and Oncology",
issn = "0167-8140",
publisher = "ELSEVIER IRELAND LTD",
number = "3",

}

RIS

TY - JOUR

T1 - Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells

AU - Maimets, Martti

AU - Bron, Reinier

AU - de Haan, Gerald

AU - van Os, Ronald

AU - Coppes, Robert P.

N1 - Copyright © 2015. Published by Elsevier Ireland Ltd.

PY - 2015/9

Y1 - 2015/9

N2 - Background and purpose: Salivary gland dysfunction is a major side effect of radiotherapy for head and neck cancer patients, which in the future might be salvaged by autologous adult salivary gland stem cell (SGSC) therapy. Since frail elderly patients may have decreased activity of SGSCs, we aimed to characterize the potential of aged SGSC-population in a murine model.Materials and methods: Salivary glands and salisphere-derived cells from young and old mice were tested for CD24 and CD29 stem cell marker expression using FACS. Moreover, in vitro expansion capability and in vivo regeneration potential upon post-irradiation transplantation of young and aged SGSCs were measured.Results: An increase in CD24(hi)/CD29(hi) putative stem cells was detected in aged salivary glands albeit with a decrease in functional ability to form salispheres. However, the salispheres formed from aged mice salivary glands expressed CD24(hi)/CD29(hi) to the same extent as the ones from young mice. Moreover, following exposure to adequate growth conditions old and young SGSCs exhibited similar in vitro expansion- and in vivo regeneration potential.Conclusions: Aged SGSCs although reduced in number are in vitro indistinguishable from young SGSCs and could potentially be used to ameliorate age- or treatment related salivary gland dysfunction. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

AB - Background and purpose: Salivary gland dysfunction is a major side effect of radiotherapy for head and neck cancer patients, which in the future might be salvaged by autologous adult salivary gland stem cell (SGSC) therapy. Since frail elderly patients may have decreased activity of SGSCs, we aimed to characterize the potential of aged SGSC-population in a murine model.Materials and methods: Salivary glands and salisphere-derived cells from young and old mice were tested for CD24 and CD29 stem cell marker expression using FACS. Moreover, in vitro expansion capability and in vivo regeneration potential upon post-irradiation transplantation of young and aged SGSCs were measured.Results: An increase in CD24(hi)/CD29(hi) putative stem cells was detected in aged salivary glands albeit with a decrease in functional ability to form salispheres. However, the salispheres formed from aged mice salivary glands expressed CD24(hi)/CD29(hi) to the same extent as the ones from young mice. Moreover, following exposure to adequate growth conditions old and young SGSCs exhibited similar in vitro expansion- and in vivo regeneration potential.Conclusions: Aged SGSCs although reduced in number are in vitro indistinguishable from young SGSCs and could potentially be used to ameliorate age- or treatment related salivary gland dysfunction. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

KW - Radiation-xerostomia

KW - Salivary glands

KW - Stem cells

KW - Regeneration

KW - Aging

KW - MUSCLE

KW - TRANSPLANTATION

U2 - 10.1016/j.radonc.2015.06.022

DO - 10.1016/j.radonc.2015.06.022

M3 - Article

VL - 116

SP - 443

EP - 448

JO - Radiotherapy and Oncology

JF - Radiotherapy and Oncology

SN - 0167-8140

IS - 3

ER -

ID: 21128615