Publication

Self-Segregation of Myelin Membrane Lipids in Model Membranes

Yurlova, L., Kahya, N., Aggarwal, S., Kaiser, H-J., Chiantia, S., Bakhti, M., Pewzner-Jung, Y., Ben-David, O., Futerman, A. H., Bruegger, B. & Simons, M., 7-Dec-2011, In : Biophysical Journal. 101, 11, p. 2713-2720 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Larisa Yurlova
  • Nicoletta Kahya
  • Shweta Aggarwal
  • Hermann-Josef Kaiser
  • Salvatore Chiantia
  • Mostafa Bakhti
  • Yael Pewzner-Jung
  • Oshrit Ben-David
  • Anthony H. Futerman
  • Britta Bruegger
  • Mikael Simons

Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed the biophysical properties of myelin membranes prepared from wild-type mice and from mouse mutants that are unable to form stable myelin. Using C-Laurdan and fluorescence correlation spectroscopy, we find that lipids are tightly organized and highly ordered in myelin isolated from wild-type mice, but not from shiverer and ceramide synthase 2 null mice. Furthermore, only myelin lipids from wild-type mice laterally segregate into physically distinct lipid phases in giant unilamellar vesicles in a process that requires very long chain glycosphingolipids. Taken together, our findings suggest that oligodendrocytes exploit the potential of lipids to self-segregate to generate a highly ordered membrane for electrical insulation of axons.

Original languageEnglish
Pages (from-to)2713-2720
Number of pages8
JournalBiophysical Journal
Volume101
Issue number11
Publication statusPublished - 7-Dec-2011

    Keywords

  • AIR-WATER-INTERFACE, CENTRAL-NERVOUS-SYSTEM, CERAMIDE SYNTHASE 2, BASIC-PROTEIN, PLASMA-MEMBRANES, CELL-SURFACE, MONOLAYERS, DOMAINS, OLIGODENDROCYTE, CHOLESTEROL

ID: 5459504