Publication

Selection, addiction & catalysis: emerging trends for the incorporation of non‐canonical amino acids into peptides and proteins in vivo

Mayer, C., 8-Jan-2019, In : ChemBioChem.

Research output: Contribution to journalReview articleAcademicpeer-review

Copy link to clipboard

Documents

  • Selection, a ddiction & c atalysis: e merging trends for the incorporation of non - canonical amino acids into peptides and proteins in vivo

    Proof, 1 MB, PDF-document

    Request copy

Links

DOI

Expanding the genetic code of organisms by incorporating non‐canonical amino acids (ncAAs) into target proteins through the suppression of stop codons in vivo has profoundly impacted how we perform protein modification or detect proteins and their interaction partners in their native environment. Yet, with genetic code expansion strategies maturing over the past 15 years, new applications that make use – or indeed repurpose – these techniques are beginning to emerge. This concept article highlights three of these developments: (1) the incorporation of ncAAs for the biosynthesis and selection of bioactive macrocyclic peptides with novel ring architectures, (2) synthetic biocontainment strategies based on the addiction of microorganisms to ncAAs, and (3) enzyme design strategies, in which ncAAs with unique functionalities enable the catalysis of new‐to‐nature reactions. Key advances in all three areas are presented and potential future applications discussed.
Original languageEnglish
JournalChemBioChem
Publication statusE-pub ahead of print - 8-Jan-2019

View graph of relations

ID: 73787007