Publication

Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site

Piquet, A. M. -T., Bolhuis, H., Davidson, A. T. & Buma, A. G. J., Jul-2010, In : FEMS Microbial Ecology. 73, 1, p. 68-82 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Piquet, A. M. -T., Bolhuis, H., Davidson, A. T., & Buma, A. G. J. (2010). Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site. FEMS Microbial Ecology, 73(1), 68-82. https://doi.org/10.1111/j.1574-6941.2010.00882.x

Author

Piquet, Anouk M. -T. ; Bolhuis, Henk ; Davidson, Andrew T. ; Buma, Anita G. J. / Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site. In: FEMS Microbial Ecology. 2010 ; Vol. 73, No. 1. pp. 68-82.

Harvard

Piquet, AM-T, Bolhuis, H, Davidson, AT & Buma, AGJ 2010, 'Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site', FEMS Microbial Ecology, vol. 73, no. 1, pp. 68-82. https://doi.org/10.1111/j.1574-6941.2010.00882.x

Standard

Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site. / Piquet, Anouk M. -T.; Bolhuis, Henk; Davidson, Andrew T.; Buma, Anita G. J.

In: FEMS Microbial Ecology, Vol. 73, No. 1, 07.2010, p. 68-82.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Piquet AM-T, Bolhuis H, Davidson AT, Buma AGJ. Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site. FEMS Microbial Ecology. 2010 Jul;73(1):68-82. https://doi.org/10.1111/j.1574-6941.2010.00882.x


BibTeX

@article{953381ebc8e14da6b8b41168933bd212,
title = "Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site",
abstract = "Despite extensive microbial biodiversity studies around the globe, studies focusing on diversity and community composition of Bacteria in Antarctic coastal regions are still scarce. Here, we studied the diversity and development of bacterioplankton communities from Prydz Bay (Eastern Antarctic) during spring and early summer 2002-2003. Additionally, we investigated the possible shaping effects of solar UV radiation (UV-R: 280-400 nm) on bacterioplankton communities incubated for 13-14 days in 650-L minicosm tanks. Ribosomal DNA sequence analysis of the natural bacterioplankton communities revealed an initial springtime community composed of three evenly abundant bacterial classes: Cytophaga-Flavobacteria- Bacteroidetes (CFB), Gammaproteobacteria and Alphaproteobacteria. At the end of spring, a shift occurred toward a CFB-dominated community, most likely a response to the onset of a springtime phytoplankton bloom. The tail end of Prydz Bay clone library diversity revealed sequences related to Deltaproteobacteria, Verrucomicrobiales, Planctomycetes, Gemmatimonadetes and an unclassified bacterium (ANT4E12). Minicosm experiments showed that incubation time was the principal determinant of bacterial community composition and that UV-R treatment significantly changed the composition in only two of the four experiments. Thus, the successional maturity of the microbial community in our minicosm studies appears to be a greater determinant of bacterial community composition rather than the nonprofound and subtle effects of UV-R.",
keywords = "Eastern Antarctic, polar, bacteria, 16S rRNA gene, DGGE, sequencing, GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, BACTERIAL COMMUNITIES, GENETIC DIVERSITY, PHYLOGENETIC COMPOSITION, SARGASSO SEA, RADIATION, OCEAN, ASSEMBLAGES, PHYTOPLANKTON",
author = "Piquet, {Anouk M. -T.} and Henk Bolhuis and Davidson, {Andrew T.} and Buma, {Anita G. J.}",
year = "2010",
month = "7",
doi = "10.1111/j.1574-6941.2010.00882.x",
language = "English",
volume = "73",
pages = "68--82",
journal = "FEMS Microbial Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site

AU - Piquet, Anouk M. -T.

AU - Bolhuis, Henk

AU - Davidson, Andrew T.

AU - Buma, Anita G. J.

PY - 2010/7

Y1 - 2010/7

N2 - Despite extensive microbial biodiversity studies around the globe, studies focusing on diversity and community composition of Bacteria in Antarctic coastal regions are still scarce. Here, we studied the diversity and development of bacterioplankton communities from Prydz Bay (Eastern Antarctic) during spring and early summer 2002-2003. Additionally, we investigated the possible shaping effects of solar UV radiation (UV-R: 280-400 nm) on bacterioplankton communities incubated for 13-14 days in 650-L minicosm tanks. Ribosomal DNA sequence analysis of the natural bacterioplankton communities revealed an initial springtime community composed of three evenly abundant bacterial classes: Cytophaga-Flavobacteria- Bacteroidetes (CFB), Gammaproteobacteria and Alphaproteobacteria. At the end of spring, a shift occurred toward a CFB-dominated community, most likely a response to the onset of a springtime phytoplankton bloom. The tail end of Prydz Bay clone library diversity revealed sequences related to Deltaproteobacteria, Verrucomicrobiales, Planctomycetes, Gemmatimonadetes and an unclassified bacterium (ANT4E12). Minicosm experiments showed that incubation time was the principal determinant of bacterial community composition and that UV-R treatment significantly changed the composition in only two of the four experiments. Thus, the successional maturity of the microbial community in our minicosm studies appears to be a greater determinant of bacterial community composition rather than the nonprofound and subtle effects of UV-R.

AB - Despite extensive microbial biodiversity studies around the globe, studies focusing on diversity and community composition of Bacteria in Antarctic coastal regions are still scarce. Here, we studied the diversity and development of bacterioplankton communities from Prydz Bay (Eastern Antarctic) during spring and early summer 2002-2003. Additionally, we investigated the possible shaping effects of solar UV radiation (UV-R: 280-400 nm) on bacterioplankton communities incubated for 13-14 days in 650-L minicosm tanks. Ribosomal DNA sequence analysis of the natural bacterioplankton communities revealed an initial springtime community composed of three evenly abundant bacterial classes: Cytophaga-Flavobacteria- Bacteroidetes (CFB), Gammaproteobacteria and Alphaproteobacteria. At the end of spring, a shift occurred toward a CFB-dominated community, most likely a response to the onset of a springtime phytoplankton bloom. The tail end of Prydz Bay clone library diversity revealed sequences related to Deltaproteobacteria, Verrucomicrobiales, Planctomycetes, Gemmatimonadetes and an unclassified bacterium (ANT4E12). Minicosm experiments showed that incubation time was the principal determinant of bacterial community composition and that UV-R treatment significantly changed the composition in only two of the four experiments. Thus, the successional maturity of the microbial community in our minicosm studies appears to be a greater determinant of bacterial community composition rather than the nonprofound and subtle effects of UV-R.

KW - Eastern Antarctic

KW - polar

KW - bacteria

KW - 16S rRNA gene

KW - DGGE

KW - sequencing

KW - GRADIENT GEL-ELECTROPHORESIS

KW - 16S RIBOSOMAL-RNA

KW - BACTERIAL COMMUNITIES

KW - GENETIC DIVERSITY

KW - PHYLOGENETIC COMPOSITION

KW - SARGASSO SEA

KW - RADIATION

KW - OCEAN

KW - ASSEMBLAGES

KW - PHYTOPLANKTON

U2 - 10.1111/j.1574-6941.2010.00882.x

DO - 10.1111/j.1574-6941.2010.00882.x

M3 - Article

VL - 73

SP - 68

EP - 82

JO - FEMS Microbial Ecology

JF - FEMS Microbial Ecology

SN - 0168-6496

IS - 1

ER -

ID: 5097532