Screening of YAC clones and building a map of the chromosome 13 region often deleted during chronic B-cell lymphocytic leucosisBrodyanskii, VM., Sulimova, GE., Udina, IG., Aitova, SS., Shaikhaev, GO., Sharikova, OA., Zakharev, VM., Fedorova, LI., Zelenin, AV., Eikhorn, S., Baush, C., Laland, M., Ross, M. & Yankovskii, NK., 1995, In : Molecular biology. 29, 5, p. 665-672 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Pools of YAC clones from the ICRF library were analyzed by PCR using PBKpt, MGG15, and D13S25 markers that flank the chromosome 13 region often deleted during chronic lymphocytic leukemia. Ten clones were found and described. Nine mega-YAC clones from the CEPH library flanking the region of interest were analyzed by PCR, which allowed building a map from 18 overlapping YAC clones and establishing the mutual arrangement of 10 tested STS markers within the 13q14.3 region (seven of those being polymorphic microsatellite sites). The region where CLL-specific rearrangements occur is not longer than 1400 bp. The centromere-proximal end of the map overlaps with the published map that includes RBI gene (retinoblastoma), while the telomere-proximal end overlaps with the described one around the WD gene (Wilson-Konovalov disease). The total length of the united map of chromosome 13 segment is about 8 million bp.
|Number of pages||8|
|Publication status||Published - 1995|
- screening, YAC contiguity, human chromosome 13, FISH, 13q14.3, D13S25, LARGE INSERTS, HUMAN GENOME, YEAST, GENE, LOCUS, DNA, LIBRARIES, CLONING, CONSTRUCTION, FRAGMENTS