Publication

Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58

Veldman, J., Visser, L., Huberts-Kregel, M., Muller, N., Hepkema, B., Van den Berg, A. & Diepstra, A., 26-Jun-2020, In : Blood. 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Veldman, J., Visser, L., Huberts-Kregel, M., Muller, N., Hepkema, B., Van den Berg, A., & Diepstra, A. (2020). Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58. Blood. https://doi.org/10.1182/blood.2020005546

Author

Veldman, Johanna ; Visser, Lydia ; Huberts-Kregel, Magdalena ; Muller, Natasja ; Hepkema, Bouke ; Van den Berg, Anke ; Diepstra, Arjan. / Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58. In: Blood. 2020.

Harvard

Veldman, J, Visser, L, Huberts-Kregel, M, Muller, N, Hepkema, B, Van den Berg, A & Diepstra, A 2020, 'Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58', Blood. https://doi.org/10.1182/blood.2020005546

Standard

Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58. / Veldman, Johanna; Visser, Lydia; Huberts-Kregel, Magdalena; Muller, Natasja; Hepkema, Bouke; Van den Berg, Anke; Diepstra, Arjan.

In: Blood, 26.06.2020.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Veldman J, Visser L, Huberts-Kregel M, Muller N, Hepkema B, Van den Berg A et al. Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58. Blood. 2020 Jun 26. https://doi.org/10.1182/blood.2020005546


BibTeX

@article{cf216236c43b4236935de56bf829ee78,
title = "Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58",
abstract = "A unique feature of Hodgkin lymphoma (HL) is the presence of CD4+ T cells that surround, protect and promote survival of tumor cells. The adhesion molecules involved in this so-called T cell rosetting are important components of the immunological synapse (IS). However, it is unknown whether this synapse is fully assembled and leads to T cell activation by enabling interaction between the T cell receptor (TCR) and human leukocyte antigen class II (HLA-II). We established a novel rosetting model by co-culturing HLA-II matched PBMCs with HL cell lines and show IS formation with activation of rosetting T cells. HLA-II downregulation by CIITA-knockout did not affect the extent of rosetting, but almost completely abrogated T cell activation. Intriguingly, the level of CD58 expression correlated with the extent of rosette formation and CD58-knockout or CD2 blockade reduced both rosette formation and T cell activation. Extension of our findings to primary HL tissue by immunohistochemistry and proximity ligation assays showed interaction of CD2 with CD58 and TCR-associated CD4 with HLA-II. In conclusion, T cell rosetting in HL is established by formation of the IS and activation of rosetting T cells critically depends on both TCR-HLA-II and CD2-CD58 interaction.",
author = "Johanna Veldman and Lydia Visser and Magdalena Huberts-Kregel and Natasja Muller and Bouke Hepkema and {Van den Berg}, Anke and Arjan Diepstra",
note = "Copyright {\circledC} 2020 American Society of Hematology.",
year = "2020",
month = "6",
day = "26",
doi = "10.1182/blood.2020005546",
language = "English",
journal = "Blood",
issn = "0006-4971",
publisher = "AMER SOC HEMATOLOGY",

}

RIS

TY - JOUR

T1 - Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58

AU - Veldman, Johanna

AU - Visser, Lydia

AU - Huberts-Kregel, Magdalena

AU - Muller, Natasja

AU - Hepkema, Bouke

AU - Van den Berg, Anke

AU - Diepstra, Arjan

N1 - Copyright © 2020 American Society of Hematology.

PY - 2020/6/26

Y1 - 2020/6/26

N2 - A unique feature of Hodgkin lymphoma (HL) is the presence of CD4+ T cells that surround, protect and promote survival of tumor cells. The adhesion molecules involved in this so-called T cell rosetting are important components of the immunological synapse (IS). However, it is unknown whether this synapse is fully assembled and leads to T cell activation by enabling interaction between the T cell receptor (TCR) and human leukocyte antigen class II (HLA-II). We established a novel rosetting model by co-culturing HLA-II matched PBMCs with HL cell lines and show IS formation with activation of rosetting T cells. HLA-II downregulation by CIITA-knockout did not affect the extent of rosetting, but almost completely abrogated T cell activation. Intriguingly, the level of CD58 expression correlated with the extent of rosette formation and CD58-knockout or CD2 blockade reduced both rosette formation and T cell activation. Extension of our findings to primary HL tissue by immunohistochemistry and proximity ligation assays showed interaction of CD2 with CD58 and TCR-associated CD4 with HLA-II. In conclusion, T cell rosetting in HL is established by formation of the IS and activation of rosetting T cells critically depends on both TCR-HLA-II and CD2-CD58 interaction.

AB - A unique feature of Hodgkin lymphoma (HL) is the presence of CD4+ T cells that surround, protect and promote survival of tumor cells. The adhesion molecules involved in this so-called T cell rosetting are important components of the immunological synapse (IS). However, it is unknown whether this synapse is fully assembled and leads to T cell activation by enabling interaction between the T cell receptor (TCR) and human leukocyte antigen class II (HLA-II). We established a novel rosetting model by co-culturing HLA-II matched PBMCs with HL cell lines and show IS formation with activation of rosetting T cells. HLA-II downregulation by CIITA-knockout did not affect the extent of rosetting, but almost completely abrogated T cell activation. Intriguingly, the level of CD58 expression correlated with the extent of rosette formation and CD58-knockout or CD2 blockade reduced both rosette formation and T cell activation. Extension of our findings to primary HL tissue by immunohistochemistry and proximity ligation assays showed interaction of CD2 with CD58 and TCR-associated CD4 with HLA-II. In conclusion, T cell rosetting in HL is established by formation of the IS and activation of rosetting T cells critically depends on both TCR-HLA-II and CD2-CD58 interaction.

U2 - 10.1182/blood.2020005546

DO - 10.1182/blood.2020005546

M3 - Article

C2 - 32589698

JO - Blood

JF - Blood

SN - 0006-4971

ER -

ID: 128399554