Publication

Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts

Westra, J., Brouwer, E., Bouwman, E., Doornbos-van der Meer, B., Posthumus, M. D., van Leeuwen, M. A., Limburg, P. C., Ueda, Y. & Kallenberg, C. G. M., 2009, CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. Shoenfeld, Y. & Gershwin, ME. (eds.). MALDEN: Blackwell Publishing, p. 706-711 6 p. (Annals of the New York Academy of Sciences; vol. 1173).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

APA

Westra, J., Brouwer, E., Bouwman, E., Doornbos-van der Meer, B., Posthumus, M. D., van Leeuwen, M. A., ... Kallenberg, C. G. M. (2009). Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts. In Y. Shoenfeld, & ME. Gershwin (Eds.), CONTEMPORARY CHALLENGES IN AUTOIMMUNITY (pp. 706-711). (Annals of the New York Academy of Sciences; Vol. 1173). MALDEN: Blackwell Publishing. https://doi.org/10.1111/j.1749-6632.2009.04736.x

Author

Westra, Johanna ; Brouwer, Elisabeth ; Bouwman, Erwin ; Doornbos-van der Meer, Berber ; Posthumus, Marcel D. ; van Leeuwen, Miek A. ; Limburg, Pieter C. ; Ueda, Yutaka ; Kallenberg, Cees G. M. / Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts. CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. editor / Y Shoenfeld ; ME Gershwin. MALDEN : Blackwell Publishing, 2009. pp. 706-711 (Annals of the New York Academy of Sciences).

Harvard

Westra, J, Brouwer, E, Bouwman, E, Doornbos-van der Meer, B, Posthumus, MD, van Leeuwen, MA, Limburg, PC, Ueda, Y & Kallenberg, CGM 2009, Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts. in Y Shoenfeld & ME Gershwin (eds), CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. Annals of the New York Academy of Sciences, vol. 1173, Blackwell Publishing, MALDEN, pp. 706-711, 6th International Congress on Autoimmunity, Portugal, 10/09/2008. https://doi.org/10.1111/j.1749-6632.2009.04736.x

Standard

Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts. / Westra, Johanna; Brouwer, Elisabeth; Bouwman, Erwin; Doornbos-van der Meer, Berber; Posthumus, Marcel D.; van Leeuwen, Miek A.; Limburg, Pieter C.; Ueda, Yutaka; Kallenberg, Cees G. M.

CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. ed. / Y Shoenfeld; ME Gershwin. MALDEN : Blackwell Publishing, 2009. p. 706-711 (Annals of the New York Academy of Sciences; Vol. 1173).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Vancouver

Westra J, Brouwer E, Bouwman E, Doornbos-van der Meer B, Posthumus MD, van Leeuwen MA et al. Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts. In Shoenfeld Y, Gershwin ME, editors, CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. MALDEN: Blackwell Publishing. 2009. p. 706-711. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.04736.x


BibTeX

@inproceedings{548d3bccd80248e481ef1e10a027f9bb,
title = "Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts",
abstract = "Background and aims: The present study was designed to investigate the effects of a novel CaMKII inhibitor SMP-114, which was effective in a collagen-induced arthritis model in rats, on rheumatoid synovial fibroblasts. Methods: Rheumatoid synovial fibroblasts (RSF) were cultured under pro-inflammatory (IL-1 beta stimulation) and hypoxic conditions. Protein and mRNA expression of HIF-1 alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed. Results: Stimulation under hypoxic conditions induced higher expression of HIF-1 alpha and VEGF mRNA than treatment with either IL-1 beta or hypoxia alone. However, incubation with a specific CaMKII inhibitor did not result in reduced VEGF production in RSF. Conclusions: CaMKII activation has been reported to be involved in HIF-1 alpha stabilization, but incubation with SMP-114, a specific CaMKII inhibitor, had no effect on HIF-1-induced VEGF production by rheumatoid synovial fibroblasts.",
keywords = "CaMKII, synovial fibroblast, VEGF, PROTEIN-KINASE-II, HYPOXIA, ACTIVATION, ANGIOGENESIS, MACROPHAGES, EXPRESSION, CYTOKINE, CELLS",
author = "Johanna Westra and Elisabeth Brouwer and Erwin Bouwman and {Doornbos-van der Meer}, Berber and Posthumus, {Marcel D.} and {van Leeuwen}, {Miek A.} and Limburg, {Pieter C.} and Yutaka Ueda and Kallenberg, {Cees G. M.}",
year = "2009",
doi = "10.1111/j.1749-6632.2009.04736.x",
language = "English",
isbn = "978-1-57331-762-7",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing",
pages = "706--711",
editor = "Y Shoenfeld and ME Gershwin",
booktitle = "CONTEMPORARY CHALLENGES IN AUTOIMMUNITY",

}

RIS

TY - GEN

T1 - Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts

AU - Westra, Johanna

AU - Brouwer, Elisabeth

AU - Bouwman, Erwin

AU - Doornbos-van der Meer, Berber

AU - Posthumus, Marcel D.

AU - van Leeuwen, Miek A.

AU - Limburg, Pieter C.

AU - Ueda, Yutaka

AU - Kallenberg, Cees G. M.

PY - 2009

Y1 - 2009

N2 - Background and aims: The present study was designed to investigate the effects of a novel CaMKII inhibitor SMP-114, which was effective in a collagen-induced arthritis model in rats, on rheumatoid synovial fibroblasts. Methods: Rheumatoid synovial fibroblasts (RSF) were cultured under pro-inflammatory (IL-1 beta stimulation) and hypoxic conditions. Protein and mRNA expression of HIF-1 alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed. Results: Stimulation under hypoxic conditions induced higher expression of HIF-1 alpha and VEGF mRNA than treatment with either IL-1 beta or hypoxia alone. However, incubation with a specific CaMKII inhibitor did not result in reduced VEGF production in RSF. Conclusions: CaMKII activation has been reported to be involved in HIF-1 alpha stabilization, but incubation with SMP-114, a specific CaMKII inhibitor, had no effect on HIF-1-induced VEGF production by rheumatoid synovial fibroblasts.

AB - Background and aims: The present study was designed to investigate the effects of a novel CaMKII inhibitor SMP-114, which was effective in a collagen-induced arthritis model in rats, on rheumatoid synovial fibroblasts. Methods: Rheumatoid synovial fibroblasts (RSF) were cultured under pro-inflammatory (IL-1 beta stimulation) and hypoxic conditions. Protein and mRNA expression of HIF-1 alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed. Results: Stimulation under hypoxic conditions induced higher expression of HIF-1 alpha and VEGF mRNA than treatment with either IL-1 beta or hypoxia alone. However, incubation with a specific CaMKII inhibitor did not result in reduced VEGF production in RSF. Conclusions: CaMKII activation has been reported to be involved in HIF-1 alpha stabilization, but incubation with SMP-114, a specific CaMKII inhibitor, had no effect on HIF-1-induced VEGF production by rheumatoid synovial fibroblasts.

KW - CaMKII

KW - synovial fibroblast

KW - VEGF

KW - PROTEIN-KINASE-II

KW - HYPOXIA

KW - ACTIVATION

KW - ANGIOGENESIS

KW - MACROPHAGES

KW - EXPRESSION

KW - CYTOKINE

KW - CELLS

U2 - 10.1111/j.1749-6632.2009.04736.x

DO - 10.1111/j.1749-6632.2009.04736.x

M3 - Conference contribution

SN - 978-1-57331-762-7

T3 - Annals of the New York Academy of Sciences

SP - 706

EP - 711

BT - CONTEMPORARY CHALLENGES IN AUTOIMMUNITY

A2 - Shoenfeld, Y

A2 - Gershwin, ME

PB - Blackwell Publishing

CY - MALDEN

ER -

ID: 4969585