Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial FibroblastsWestra, J., Brouwer, E., Bouwman, E., Doornbos-van der Meer, B., Posthumus, M. D., van Leeuwen, M. A., Limburg, P. C., Ueda, Y. & Kallenberg, C. G. M., 2009, CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. Shoenfeld, Y. & Gershwin, ME. (eds.). MALDEN: Blackwell Publishing, p. 706-711 6 p. (Annals of the New York Academy of Sciences; vol. 1173).
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review
Background and aims: The present study was designed to investigate the effects of a novel CaMKII inhibitor SMP-114, which was effective in a collagen-induced arthritis model in rats, on rheumatoid synovial fibroblasts. Methods: Rheumatoid synovial fibroblasts (RSF) were cultured under pro-inflammatory (IL-1 beta stimulation) and hypoxic conditions. Protein and mRNA expression of HIF-1 alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed. Results: Stimulation under hypoxic conditions induced higher expression of HIF-1 alpha and VEGF mRNA than treatment with either IL-1 beta or hypoxia alone. However, incubation with a specific CaMKII inhibitor did not result in reduced VEGF production in RSF. Conclusions: CaMKII activation has been reported to be involved in HIF-1 alpha stabilization, but incubation with SMP-114, a specific CaMKII inhibitor, had no effect on HIF-1-induced VEGF production by rheumatoid synovial fibroblasts.
|Title of host publication||CONTEMPORARY CHALLENGES IN AUTOIMMUNITY|
|Editors||Y Shoenfeld, ME Gershwin|
|Place of Publication||MALDEN|
|Number of pages||6|
|Publication status||Published - 2009|
|Event||6th International Congress on Autoimmunity - , Portugal|
Duration: 10-Sep-2008 → 14-Sep-2008
|Name||Annals of the New York Academy of Sciences|
|Other||6th International Congress on Autoimmunity|
|Period||10/09/2008 → 14/09/2008|
6th International Congress on Autoimmunity
10/09/2008 → 14/09/2008Portugal
- CaMKII, synovial fibroblast, VEGF, PROTEIN-KINASE-II, HYPOXIA, ACTIVATION, ANGIOGENESIS, MACROPHAGES, EXPRESSION, CYTOKINE, CELLS