Publication

Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure

Vegter, E. L., Ovchinnikova, E. S., Sillje, H. H. W., Meems, L. M. G., van der Pol, A., van der Velde, A. R., Berezikov, E., Voors, A. A., de Boer, R. A. & van der Meer, P., 5-May-2017, In : PLoS ONE. 12, 5, 14 p., e0177242.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Vegter, E. L., Ovchinnikova, E. S., Sillje, H. H. W., Meems, L. M. G., van der Pol, A., van der Velde, A. R., Berezikov, E., Voors, A. A., de Boer, R. A., & van der Meer, P. (2017). Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure. PLoS ONE, 12(5), [e0177242]. https://doi.org/10.1371/journal.pone.0177242

Author

Vegter, Eline L. ; Ovchinnikova, Ekaterina S. ; Sillje, Herman H. W. ; Meems, Laura M. G. ; van der Pol, Atze ; van der Velde, A. Rogier ; Berezikov, Eugene ; Voors, Adriaan A. ; de Boer, Rudolf A. ; van der Meer, Peter. / Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure. In: PLoS ONE. 2017 ; Vol. 12, No. 5.

Harvard

Vegter, EL, Ovchinnikova, ES, Sillje, HHW, Meems, LMG, van der Pol, A, van der Velde, AR, Berezikov, E, Voors, AA, de Boer, RA & van der Meer, P 2017, 'Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure', PLoS ONE, vol. 12, no. 5, e0177242. https://doi.org/10.1371/journal.pone.0177242

Standard

Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure. / Vegter, Eline L.; Ovchinnikova, Ekaterina S.; Sillje, Herman H. W.; Meems, Laura M. G.; van der Pol, Atze; van der Velde, A. Rogier; Berezikov, Eugene; Voors, Adriaan A.; de Boer, Rudolf A.; van der Meer, Peter.

In: PLoS ONE, Vol. 12, No. 5, e0177242, 05.05.2017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Vegter EL, Ovchinnikova ES, Sillje HHW, Meems LMG, van der Pol A, van der Velde AR et al. Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure. PLoS ONE. 2017 May 5;12(5). e0177242. https://doi.org/10.1371/journal.pone.0177242


BibTeX

@article{886e791ac5fe4a779088b03574e77ca8,
title = "Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure",
abstract = "IntroductionWe recently identified a set of plasma microRNAs (miRNAs) that are downregulated in patients with heart failure in comparison with control subjects. To better understand their meaning and function, we sought to validate these circulating miRNAs in 3 different well-established rat and mouse heart failure models, and correlated the miRNAs to parameters of cardiac function.MethodsThe previously identified let-7i-5p, miR-16-5p, miR-18a-5p, miR-26b-5p, miR-27a-3p, miR30e-5p, miR-199a-3p, miR-223-3p, miR-423-3p, miR-423-5p and miR-652-3p were measured by means of quantitative real time polymerase chain reaction (qRT-PCR) in plasma samples of 8 homozygous TGR(mREN2)27 (Ren2) transgenic rats and 8 (control) Sprague-Dawley rats, 6 mice with angiotensin II-induced heart failure (AngII) and 6 control mice, and 8 mice with ischemic heart failure and 6 controls. Circulating miRNA levels were compared between the heart failure animals and healthy controls.ResultsRen2 rats, AngII mice and mice with ischemic heart failure showed clear signs of heart failure, exemplified by increased left ventricular and lung weights, elevated end-diastolic left ventricular pressures, increased expression of cardiac stress markers and reduced left ventricular ejection fraction. All miRNAs were detectable in plasma from rats and mice. No significant differences were observed between the circulating miRNAs in heart failure animals when compared to the healthy controls (all P>0.05) and no robust associations with cardiac function could be found.ConclusionsThe previous observation that miRNAs circulate in lower levels in human patients with heart failure could not be validated in well-established rat and mouse heart failure models. These results question the translation of data on human circulating miRNA levels to experimental models, and vice versa the validity of experimental miRNA data for human heart failure.",
keywords = "CARDIAC-HYPERTROPHY, BIOMARKERS, HYPERTENSION, PROGRESSION, DISEASE, INJURY, KINASE",
author = "Vegter, {Eline L.} and Ovchinnikova, {Ekaterina S.} and Sillje, {Herman H. W.} and Meems, {Laura M. G.} and {van der Pol}, Atze and {van der Velde}, {A. Rogier} and Eugene Berezikov and Voors, {Adriaan A.} and {de Boer}, {Rudolf A.} and {van der Meer}, Peter",
year = "2017",
month = may,
day = "5",
doi = "10.1371/journal.pone.0177242",
language = "English",
volume = "12",
journal = "PLOS-One",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "5",

}

RIS

TY - JOUR

T1 - Rodent heart failure models do not reflect the human circulating microRNA signature in heart failure

AU - Vegter, Eline L.

AU - Ovchinnikova, Ekaterina S.

AU - Sillje, Herman H. W.

AU - Meems, Laura M. G.

AU - van der Pol, Atze

AU - van der Velde, A. Rogier

AU - Berezikov, Eugene

AU - Voors, Adriaan A.

AU - de Boer, Rudolf A.

AU - van der Meer, Peter

PY - 2017/5/5

Y1 - 2017/5/5

N2 - IntroductionWe recently identified a set of plasma microRNAs (miRNAs) that are downregulated in patients with heart failure in comparison with control subjects. To better understand their meaning and function, we sought to validate these circulating miRNAs in 3 different well-established rat and mouse heart failure models, and correlated the miRNAs to parameters of cardiac function.MethodsThe previously identified let-7i-5p, miR-16-5p, miR-18a-5p, miR-26b-5p, miR-27a-3p, miR30e-5p, miR-199a-3p, miR-223-3p, miR-423-3p, miR-423-5p and miR-652-3p were measured by means of quantitative real time polymerase chain reaction (qRT-PCR) in plasma samples of 8 homozygous TGR(mREN2)27 (Ren2) transgenic rats and 8 (control) Sprague-Dawley rats, 6 mice with angiotensin II-induced heart failure (AngII) and 6 control mice, and 8 mice with ischemic heart failure and 6 controls. Circulating miRNA levels were compared between the heart failure animals and healthy controls.ResultsRen2 rats, AngII mice and mice with ischemic heart failure showed clear signs of heart failure, exemplified by increased left ventricular and lung weights, elevated end-diastolic left ventricular pressures, increased expression of cardiac stress markers and reduced left ventricular ejection fraction. All miRNAs were detectable in plasma from rats and mice. No significant differences were observed between the circulating miRNAs in heart failure animals when compared to the healthy controls (all P>0.05) and no robust associations with cardiac function could be found.ConclusionsThe previous observation that miRNAs circulate in lower levels in human patients with heart failure could not be validated in well-established rat and mouse heart failure models. These results question the translation of data on human circulating miRNA levels to experimental models, and vice versa the validity of experimental miRNA data for human heart failure.

AB - IntroductionWe recently identified a set of plasma microRNAs (miRNAs) that are downregulated in patients with heart failure in comparison with control subjects. To better understand their meaning and function, we sought to validate these circulating miRNAs in 3 different well-established rat and mouse heart failure models, and correlated the miRNAs to parameters of cardiac function.MethodsThe previously identified let-7i-5p, miR-16-5p, miR-18a-5p, miR-26b-5p, miR-27a-3p, miR30e-5p, miR-199a-3p, miR-223-3p, miR-423-3p, miR-423-5p and miR-652-3p were measured by means of quantitative real time polymerase chain reaction (qRT-PCR) in plasma samples of 8 homozygous TGR(mREN2)27 (Ren2) transgenic rats and 8 (control) Sprague-Dawley rats, 6 mice with angiotensin II-induced heart failure (AngII) and 6 control mice, and 8 mice with ischemic heart failure and 6 controls. Circulating miRNA levels were compared between the heart failure animals and healthy controls.ResultsRen2 rats, AngII mice and mice with ischemic heart failure showed clear signs of heart failure, exemplified by increased left ventricular and lung weights, elevated end-diastolic left ventricular pressures, increased expression of cardiac stress markers and reduced left ventricular ejection fraction. All miRNAs were detectable in plasma from rats and mice. No significant differences were observed between the circulating miRNAs in heart failure animals when compared to the healthy controls (all P>0.05) and no robust associations with cardiac function could be found.ConclusionsThe previous observation that miRNAs circulate in lower levels in human patients with heart failure could not be validated in well-established rat and mouse heart failure models. These results question the translation of data on human circulating miRNA levels to experimental models, and vice versa the validity of experimental miRNA data for human heart failure.

KW - CARDIAC-HYPERTROPHY

KW - BIOMARKERS

KW - HYPERTENSION

KW - PROGRESSION

KW - DISEASE

KW - INJURY

KW - KINASE

U2 - 10.1371/journal.pone.0177242

DO - 10.1371/journal.pone.0177242

M3 - Article

C2 - 28475616

VL - 12

JO - PLOS-One

JF - PLOS-One

SN - 1932-6203

IS - 5

M1 - e0177242

ER -

ID: 46955353