RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complexChang, M., Bellaoui, M., Zhang, CY., Desai, R., Morozov, P., Delgado-Cruzata, L., Rothstein, R., Freyer, GA., Boone, C. & Brown, GW., 1-Jun-2005, In : EMBO Journal. 24, 11, p. 2024-2033 10 p.
Research output: Contribution to journal › Article › Academic › peer-review
SGS1 encodes a DNA helicase whose homologues in human cells include the BLM, WRN, and RECQ4 genes, mutations in which lead to cancer-predisposition syndromes. Clustering of synthetic genetic interactions identified by large-scale genetic network analysis revealed that the genetic interaction profile of the gene RMI1 ( RecQ-mediated genome instability, also known as NCE4 and YPL024W) was highly similar to that of SGS1 and TOP3, suggesting a functional relationship between Rmi1 and the Sgs1/Top3 complex. We show that Rmi1 physically interacts with Sgs1 and Top3 and is a third member of this complex. Cells lacking RMI1 activate the Rad53 checkpoint kinase, undergo a mitotic delay, and display increased relocalization of the recombination repair protein Rad52, indicating the presence of spontaneous DNA damage. Consistent with a role for RMI1 in maintaining genome integrity, rmi1 Delta cells exhibit increased recombination frequency and increased frequency of gross chromosomal rearrangements. In addition, rmi1 Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response.
|Number of pages||10|
|Publication status||Published - 1-Jun-2005|
- checkpoint, genome instability, RecQ helicase, Sgs1, Top3, WERNERS-SYNDROME GENES, BLOOMS-SYNDROME GENE, STRAND-BREAK REPAIR, DNA TOPOISOMERASE-III, YEAST SGS1 HELICASE, SACCHAROMYCES-CEREVISIAE, S-PHASE, HOMOLOGOUS RECOMBINATION, SCHIZOSACCHAROMYCES-POMBE, CHECKPOINT CONTROL