Rewriting DNA Methylation Signatures at Will: The Curable Genome Within Reach?

Stolzenburg, S., Goubert, D. & Rots, M. G., 2016, DNA Methyltransferases: Role and Function. Jeltsch, A. & Jurkowska, R. Z. (eds.). Springer International Publishing, Vol. 945. p. 475-490 16 p. (Advances in Experimental Medicine and Biology; vol. 945).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Epigenetic regulation of gene expression is vital for the maintenance of genome integrity and cell phenotype. In addition, many different diseases have underlying epigenetic mutations, and understanding their role and function may unravel new insights for diagnosis, treatment, and even prevention of diseases. It was an important breakthrough when epigenetic alterations could be gene-specifically manipulated using epigenetic regulatory proteins in an approach termed epigenetic editing. Epigenetic editors can be designed for virtually any gene by targeting effector domains to a preferred sequence, where they write or erase the desired epigenetic modification. This chapter describes the tools for editing DNA methylation signatures and their applications. In addition, we explain how to achieve targeted DNA (de)methylation and discuss the advantages and disadvantages of this approach. Silencing genes directly at the DNA methylation level instead of targeting the protein and/or RNA is a major improvement, as repression is achieved at the source of expression, potentially eliminating the need for continuous administration. Re-expression of silenced genes by targeted demethylation might closely represent the natural situation, in which all transcript variants might be expressed in a sustainable manner. Altogether epigenetic editing, for example, by rewriting DNA methylation, will assist in realizing the curable genome concept.

Original languageEnglish
Title of host publicationDNA Methyltransferases
Subtitle of host publicationRole and Function
EditorsAlbert Jeltsch, Renata Z. Jurkowska
PublisherSpringer International Publishing
Number of pages16
ISBN (Electronic)978-3-319-43624-1
ISBN (Print)978-3-319-43622-7
Publication statusPublished - 2016

Publication series

NameAdvances in Experimental Medicine and Biology
PublisherSpringer International Publishing
ISSN (Print)0065-2598

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