Publication

Renal expression of endothelial and inducible nitric oxide synthase, and formation of peroxynitrite-modified proteins and reactive oxygen species in Wegener's granulomatosis

Heeringa, P., Bijl, M., de Jager-Krikken, A., Zandvoort, A., Dijkstra, G., Moshage, H., Tervaert, JWC., Tiebosch, ATMG., Kallenberg, CGM. & van Goor, H., Feb-2001, In : JOURNAL OF PATHOLOGY. 193, 2, p. 224-232 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

To investigate the role of nitric oxide (NO) in glomerular inflammation, the expression of endothelial NO synthase (eNOS) and inducible NOS (iNOS) was studied in conjunction with inflammatory cell influx, H2O2 production, and the formation of nitrotyrosines in renal biopsies from patients with Wegener's granulomatosis (WG), Renal cryostat sections from patients with WG (n = 15) were stained by immunohistochemistry for eNOS, iNOS, endothelial cells (CD31), nitrotyrosines, polymorphonuclear cells (PMNs, CD15), and monocytes/macrophages (CD14, CD68), Production of H2O2 was identified by enzyme cytochemistry using diaminobenzidine. In control tissues, strong staining for eNOS was found in glomerular and interstitial tubular capillaries and cortical vessels. A significant reduction in eNOS expression was found in WG biopsies, which was associated with a reduction in CD31 expression. Expression of iNOS was found in infiltrating inflammatory cells, mainly located in the interstitium, H2O2-producing cells were detected in glomeruli and were abundantly present in the interstitium. Nitrotyrosine-positive cells, however, were almost exclusively found in the interstitium, It is concluded that renal inflammation in WG is associated with the induction of iNOS in inflammatory cells and the formation of nitrotyrosines, Expression of eNOS in glomerular capillaries is lost, most likely due to endothelial cell damage. These results suggest that decreased NO. production by endothelial cells, in conjunction with increased NO. production by iNOS-positive inflammatory cells, is involved in renal tissue injury in WG, Copyright (C) 2000 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)224-232
Number of pages9
JournalJOURNAL OF PATHOLOGY
Volume193
Issue number2
Publication statusPublished - Feb-2001

    Keywords

  • Wegener's granulomatosis, nitric oxide synthases, nitrotyrosine, oxygen radicals, RAT NEPHROTOXIC NEPHRITIS, CRESCENTIC GLOMERULONEPHRITIS, CLASSIFICATION, ACTIVATION, BLOOD, MICE

View graph of relations

ID: 2007874