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Removal of phytotoxic compounds from torrefied grass fibres by plant-beneficial microorganisms

Trifonova, R., Postma, J., Verstappen, F. W. A., Bouwmeester, H. J., Ketelaars, J. J. M. H. & van Elsas, J-D., Oct-2008, In : FEMS Microbial Ecology. 66, 1, p. 158-166 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Radoslava Trifonova
  • Joeke Postma
  • Francel W. A. Verstappen
  • Harro J. Bouwmeester
  • Jan J. M. H. Ketelaars
  • Jan-Dirk van Elsas

We aimed to select microorganisms colonizing torrefied grass fibres (TGF) and simultaneously reducing the phytotoxicity which appeared after heat treatment of the fibres. Eighty-eight bacterial strains and one fungus, previously isolated from a sequential enrichment experiment on torrefied fibres and extracts, were tested separately for their capacity to decrease phytotoxicity. Eleven of the bacterial strains and the fungus significantly reduced phytotoxicity. These organisms were checked for their ability to grow on agar containing phenol, 2-methoxyphenol, 2,6-dimethoxyphenol, 2-furalaldehyde, pyrrole-2-carboxaldehyde and furan-2-methanol as sole carbon sources. The fungus F/TGF15 and the bacterial strain 66/TGF15 were able to grow on all six compounds. Strains 15/TGE5, 23/TGE5, 43/TGE20, 56/TGF10 and 95/TGF15 grew on two to four compounds, and strain 72/TGF15 only on one compound. Strains 31/TGE5, 34/TGE5, 48/TGE20 and 70/TGF15 did not grow on any of the single toxic compounds. GC analyses of torrefied grass extracts (TGE) determined which compounds were removed by the microorganisms. F/TGF15 was the only isolate depleting phenol, 2-methoxyphenol, 2-dihydrofuranone and pyrrole-2,5-dione-3-ethyl-4-methyl. Strains 15/TGE5, 23/TGE5, 31/TGE5 and 56/TGF10, and the fungus depleted 2-furalaldehyde, 2-furan-carboxaldehyde-5-methyl, pyrrole-2-carboxaldehyde, 5-acetoxymethyl-2-furaldehyde and benzaldehyde-3-hydroxy-4-methoxy. These promising candidates for colonizing and simultaneously reducing the phytotoxicity of TGF were affiliated with Pseudomonas putida, Serratia plymuthica, Pseudomonas corrugata, Methylobacterium radiotolerans and Coniochaeta ligniaria.

Original languageEnglish
Pages (from-to)158-166
Number of pages9
JournalFEMS Microbial Ecology
Volume66
Issue number1
Publication statusPublished - Oct-2008

    Keywords

  • Pseudomonas, Serratia, Methylobacterium, Coniochaeta ligniaria, 2-furalaldehyde (furfural), phenol, MILL WASTE-WATER, ESCHERICHIA-COLI, PHENOLIC REMOVAL, LACCASE ACTIVITY, GROWTH, STRAIN, PEAT, DETOXIFICATION, FERMENTATION, ENZYME

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