Removal of Pex3p is an important initial stage in selective peroxisome degradation in Hansenula polymorphaBellu, AR., Salomons, FA., Kiel, JAKW., Veenhuis, M. & van der Klei, IJ., 8-Nov-2002, In : The Journal of Biological Chemistry. 277, 45, p. 42875-42880 6 p.
Research output: Contribution to journal › Article › Academic › peer-review
Selective degradation of peroxisomes (macropexophagy) in Hansenula polymorpha involves the sequestration of individual organelles to be degraded by membranes prior to the fusion of this compartment with the vacuole and subsequent degradation of the whole organelle by vacuolar hydrolases. Here we show that Pex3p, a peroxisomal membrane protein essential for peroxisome biogenesis, escapes this autophagic process. Upon induction of macropexophagy, Pex3p is removed from the organelle tagged for degradation prior to its sequestration. Our data indicate that Pex3p degradation is essential to allow the initiation of the organellar degradation process. Also, in a specific peroxisome degradation-deficient (pdd) mutant in which sequestration still occurs but the vacuolar fusion event is disturbed, the turnover of Pex3p is still observed. Taken together, our data suggest that degradation of Pex3p is part of the initial degradation machinery of individual peroxisomes.
|Number of pages||6|
|Journal||The Journal of Biological Chemistry|
|Publication status||Published - 8-Nov-2002|
- TRANSMEMBRANE CONDUCTANCE REGULATOR, YEAST PICHIA-PASTORIS, ASSEMBLY PROTEIN, GENE ENCODES, BIOGENESIS, MEMBRANE, PROLIFERATION, AUTOPHAGY, TURNOVER, OXIDASE