Publication

Regulation of oligodendrocyte differentiation: Protein kinase C activation prevents differentiation of O2A progenitor cells toward oligodendrocytes

Baron, W., de Jonge, JC., Vries, de, H. & Hoekstra, D., Feb-1998, In : Glia. 22, 2, p. 121-129 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

Oligodendrocytes differentiate on a specific schedule in vivo in order to myelinate axons at the precise time and at the appropriate position. The current study was undertaken to obtain further insight as to how this timed appearance is regulated intracellularly. We observed that exposure of O2A progenitor cells in culture to phorbol 12-myristate 13-acetate (PMA; an activator of protein kinase C, PKC) inhibited their differentiation to oligodendrocytes by suppressing the expression of specific myelin markers at the O4-stage. To positively identify a role of PKC per se in differentiation, the use of a minimal medium with low serum content turned out to be essential. This was demonstrated by showing that the inhibitory effect of PMA on oligodendrocyte differentiation could be completely abolished by a combined action of insulin, triiodothyronine (T3), hydrocortisone and other components of a chemically defined medium (CDM). Furthermore, the PMA-mediated inhibition of oligodendrocyte differentiation could be partially restored by activation of the cAMP signal transduction pathway. The results indicate that PKC plays a crucial role in the differentiation of O2A progenitor cells toward oligodendrocytes: PKC activation prevents differentiation of O2A progenitor cells, whereas differentiation toward oligodendrocytes is dependent on other signaling compounds which may counteract the PKC signal transduction route. (C) 1998 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalGlia
Volume22
Issue number2
Publication statusPublished - Feb-1998

    Keywords

  • protein kinase C, differentiation, oligodendrocyte, cAMP-dependent protein kinase, O2A progenitor cell, MYELIN BASIC-PROTEIN, CULTURED RAT OLIGODENDROCYTES, GROWTH FACTOR-I, GENE-EXPRESSION, PHOSPHORYLATION, PROLIFERATION, INSULIN, INHIBITION, SUBSTRATE, ASTROCYTE

ID: 3700109