Publication

Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury

Wolthuis, E. K., Vlaar, A. P. J., Choi, G., Roelofs, J. J. T. H., Haitsma, J. J., van der Poll, T., Juffermans, N. P., Zweers, M. M. & Schultz, M. J., 2009, In : Shock. 31, 3, p. 262-6 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Wolthuis, E. K., Vlaar, A. P. J., Choi, G., Roelofs, J. J. T. H., Haitsma, J. J., van der Poll, T., ... Schultz, M. J. (2009). Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury. Shock, 31(3), 262-6. https://doi.org/10.1097/SHK.0b013e31817d42dd

Author

Wolthuis, Esther K ; Vlaar, Alexander P J ; Choi, Goda ; Roelofs, Joris J T H ; Haitsma, Jack J ; van der Poll, Tom ; Juffermans, Nicole P ; Zweers, Machteld M ; Schultz, Marcus J. / Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury. In: Shock. 2009 ; Vol. 31, No. 3. pp. 262-6.

Harvard

Wolthuis, EK, Vlaar, APJ, Choi, G, Roelofs, JJTH, Haitsma, JJ, van der Poll, T, Juffermans, NP, Zweers, MM & Schultz, MJ 2009, 'Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury', Shock, vol. 31, no. 3, pp. 262-6. https://doi.org/10.1097/SHK.0b013e31817d42dd

Standard

Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury. / Wolthuis, Esther K; Vlaar, Alexander P J; Choi, Goda; Roelofs, Joris J T H; Haitsma, Jack J; van der Poll, Tom; Juffermans, Nicole P; Zweers, Machteld M; Schultz, Marcus J.

In: Shock, Vol. 31, No. 3, 2009, p. 262-6.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Wolthuis EK, Vlaar APJ, Choi G, Roelofs JJTH, Haitsma JJ, van der Poll T et al. Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury. Shock. 2009;31(3):262-6. https://doi.org/10.1097/SHK.0b013e31817d42dd


BibTeX

@article{f55918ae44bd446c8ef55d49b509b4ec,
title = "Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury",
abstract = "Ventilator-induced lung injury is mediated, at least in part, by TNF-alpha. We determined the effect of a recombinant human soluble TNF receptor fusion protein (etanercept) on mechanical ventilation (MV)-induced changes in a murine ventilator-induced lung injury model. After pretreatment with etanercept or placebo, C57Bl/6 mice were anesthetized and randomized to MV with either low tidal volumes (VT, approximately 7.5 mL/kg) or high VT ( approximately 15 mL/kg) for 5 h. Instrumented but spontaneously breathing mice served as controls. End points were lung wet-to-dry ratios, lung histopathology scores, protein levels, neutrophil cell counts and thrombin-antithrombin complex levels in bronchoalveolar lavage fluid (BALF), and cytokine levels in lung homogenates. The number of caspase 3-positive cells was used as a measure for apoptosis. Etanercept treatment attenuated MV-induced changes, in particular, in MV with high VT. Compared with placebo, etanercept reduced the number of neutrophils in BALF and thrombin-antithrombin complex levels in BALF and cytokine levels in lung homogenates. Lung wet-to-dry ratios, histopathology scores, and local protein levels in BALF, however, were not influenced by etanercept treatment. The number of caspase 3-positive cells was significantly higher in etanercept-treated animals. Inhibition of TNF by etanercept attenuates, in part, MV-induced changes.",
keywords = "Animals, Anti-Inflammatory Agents, Non-Steroidal, Bronchoalveolar Lavage Fluid, Caspase 3, Disease Models, Animal, Etanercept, Female, Humans, Immunoglobulin G, Lung, Mice, Receptors, Tumor Necrosis Factor, Recombinant Fusion Proteins, Tumor Necrosis Factor-alpha, Ventilator-Induced Lung Injury",
author = "Wolthuis, {Esther K} and Vlaar, {Alexander P J} and Goda Choi and Roelofs, {Joris J T H} and Haitsma, {Jack J} and {van der Poll}, Tom and Juffermans, {Nicole P} and Zweers, {Machteld M} and Schultz, {Marcus J}",
year = "2009",
doi = "10.1097/SHK.0b013e31817d42dd",
language = "English",
volume = "31",
pages = "262--6",
journal = "Shock",
issn = "1073-2322",
publisher = "LIPPINCOTT WILLIAMS & WILKINS",
number = "3",

}

RIS

TY - JOUR

T1 - Recombinant human soluble tumor necrosis factor-alpha receptor fusion protein partly attenuates ventilator-induced lung injury

AU - Wolthuis, Esther K

AU - Vlaar, Alexander P J

AU - Choi, Goda

AU - Roelofs, Joris J T H

AU - Haitsma, Jack J

AU - van der Poll, Tom

AU - Juffermans, Nicole P

AU - Zweers, Machteld M

AU - Schultz, Marcus J

PY - 2009

Y1 - 2009

N2 - Ventilator-induced lung injury is mediated, at least in part, by TNF-alpha. We determined the effect of a recombinant human soluble TNF receptor fusion protein (etanercept) on mechanical ventilation (MV)-induced changes in a murine ventilator-induced lung injury model. After pretreatment with etanercept or placebo, C57Bl/6 mice were anesthetized and randomized to MV with either low tidal volumes (VT, approximately 7.5 mL/kg) or high VT ( approximately 15 mL/kg) for 5 h. Instrumented but spontaneously breathing mice served as controls. End points were lung wet-to-dry ratios, lung histopathology scores, protein levels, neutrophil cell counts and thrombin-antithrombin complex levels in bronchoalveolar lavage fluid (BALF), and cytokine levels in lung homogenates. The number of caspase 3-positive cells was used as a measure for apoptosis. Etanercept treatment attenuated MV-induced changes, in particular, in MV with high VT. Compared with placebo, etanercept reduced the number of neutrophils in BALF and thrombin-antithrombin complex levels in BALF and cytokine levels in lung homogenates. Lung wet-to-dry ratios, histopathology scores, and local protein levels in BALF, however, were not influenced by etanercept treatment. The number of caspase 3-positive cells was significantly higher in etanercept-treated animals. Inhibition of TNF by etanercept attenuates, in part, MV-induced changes.

AB - Ventilator-induced lung injury is mediated, at least in part, by TNF-alpha. We determined the effect of a recombinant human soluble TNF receptor fusion protein (etanercept) on mechanical ventilation (MV)-induced changes in a murine ventilator-induced lung injury model. After pretreatment with etanercept or placebo, C57Bl/6 mice were anesthetized and randomized to MV with either low tidal volumes (VT, approximately 7.5 mL/kg) or high VT ( approximately 15 mL/kg) for 5 h. Instrumented but spontaneously breathing mice served as controls. End points were lung wet-to-dry ratios, lung histopathology scores, protein levels, neutrophil cell counts and thrombin-antithrombin complex levels in bronchoalveolar lavage fluid (BALF), and cytokine levels in lung homogenates. The number of caspase 3-positive cells was used as a measure for apoptosis. Etanercept treatment attenuated MV-induced changes, in particular, in MV with high VT. Compared with placebo, etanercept reduced the number of neutrophils in BALF and thrombin-antithrombin complex levels in BALF and cytokine levels in lung homogenates. Lung wet-to-dry ratios, histopathology scores, and local protein levels in BALF, however, were not influenced by etanercept treatment. The number of caspase 3-positive cells was significantly higher in etanercept-treated animals. Inhibition of TNF by etanercept attenuates, in part, MV-induced changes.

KW - Animals

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Bronchoalveolar Lavage Fluid

KW - Caspase 3

KW - Disease Models, Animal

KW - Etanercept

KW - Female

KW - Humans

KW - Immunoglobulin G

KW - Lung

KW - Mice

KW - Receptors, Tumor Necrosis Factor

KW - Recombinant Fusion Proteins

KW - Tumor Necrosis Factor-alpha

KW - Ventilator-Induced Lung Injury

U2 - 10.1097/SHK.0b013e31817d42dd

DO - 10.1097/SHK.0b013e31817d42dd

M3 - Article

C2 - 18650784

VL - 31

SP - 262

EP - 266

JO - Shock

JF - Shock

SN - 1073-2322

IS - 3

ER -

ID: 29061632