Publication

Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level

Marchetti, M., Kamsma, D., Cazares Vargas, E., Hernandez García, A., van der Schoot, P., de Vries, R., Wuite, G. J. L. & Roos, W. H., 14-Aug-2019, In : Nano Letters. 19, 8, p. 5746-5753 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Marchetti, M., Kamsma, D., Cazares Vargas, E., Hernandez García, A., van der Schoot, P., de Vries, R., ... Roos, W. H. (2019). Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level. Nano Letters, 19(8), 5746-5753. https://doi.org/10.1021/acs.nanolett.9b02376

Author

Marchetti, Margherita ; Kamsma, Douwe ; Cazares Vargas, Ernesto ; Hernandez García, Armando ; van der Schoot, Paul ; de Vries, Renko ; Wuite, Gijs J L ; Roos, Wouter H. / Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level. In: Nano Letters. 2019 ; Vol. 19, No. 8. pp. 5746-5753.

Harvard

Marchetti, M, Kamsma, D, Cazares Vargas, E, Hernandez García, A, van der Schoot, P, de Vries, R, Wuite, GJL & Roos, WH 2019, 'Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level', Nano Letters, vol. 19, no. 8, pp. 5746-5753. https://doi.org/10.1021/acs.nanolett.9b02376

Standard

Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level. / Marchetti, Margherita; Kamsma, Douwe; Cazares Vargas, Ernesto; Hernandez García, Armando; van der Schoot, Paul; de Vries, Renko; Wuite, Gijs J L; Roos, Wouter H.

In: Nano Letters, Vol. 19, No. 8, 14.08.2019, p. 5746-5753.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Marchetti M, Kamsma D, Cazares Vargas E, Hernandez García A, van der Schoot P, de Vries R et al. Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level. Nano Letters. 2019 Aug 14;19(8):5746-5753. https://doi.org/10.1021/acs.nanolett.9b02376


BibTeX

@article{11b4a02dabb2475ebc01f08e2cde5c46,
title = "Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level",
abstract = "While the structure of a multitude of viral particles has been resolved to atomistic detail, their assembly pathways remain largely elusive. Key unresolved issues are particle nucleation, particle growth, and the mode of genome compaction. These issues are difficult to address in bulk approaches and are effectively only accessible by the real-time tracking of assembly dynamics of individual particles. This we do here by studying the assembly into rod-shaped viruslike particles (VLPs) of artificial capsid polypeptides. Using fluorescence optical tweezers, we establish that small oligomers perform one-dimensional diffusion along the DNA. Larger oligomers are immobile and nucleate VLP growth. A multiplexed acoustic force spectroscopy approach reveals that DNA is compacted in regular steps, suggesting packaging via helical wrapping into a nucleocapsid. By reporting how real-time assembly tracking elucidates viral nucleation and growth principles, our work opens the door to a fundamental understanding of the complex assembly pathways of both VLPs and naturally evolved viruses.",
keywords = "Self-assembly, artificial virus, physical virology, biophysics, optical tweezers, acoustic force spectroscopy, OPTICAL TWEEZERS, DNA, DYNAMICS, PROTEINS, NUCLEATION, COAT",
author = "Margherita Marchetti and Douwe Kamsma and {Cazares Vargas}, Ernesto and {Hernandez Garc{\'i}a}, Armando and {van der Schoot}, Paul and {de Vries}, Renko and Wuite, {Gijs J L} and Roos, {Wouter H}",
year = "2019",
month = "8",
day = "14",
doi = "10.1021/acs.nanolett.9b02376",
language = "English",
volume = "19",
pages = "5746--5753",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "AMER CHEMICAL SOC",
number = "8",

}

RIS

TY - JOUR

T1 - Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level

AU - Marchetti, Margherita

AU - Kamsma, Douwe

AU - Cazares Vargas, Ernesto

AU - Hernandez García, Armando

AU - van der Schoot, Paul

AU - de Vries, Renko

AU - Wuite, Gijs J L

AU - Roos, Wouter H

PY - 2019/8/14

Y1 - 2019/8/14

N2 - While the structure of a multitude of viral particles has been resolved to atomistic detail, their assembly pathways remain largely elusive. Key unresolved issues are particle nucleation, particle growth, and the mode of genome compaction. These issues are difficult to address in bulk approaches and are effectively only accessible by the real-time tracking of assembly dynamics of individual particles. This we do here by studying the assembly into rod-shaped viruslike particles (VLPs) of artificial capsid polypeptides. Using fluorescence optical tweezers, we establish that small oligomers perform one-dimensional diffusion along the DNA. Larger oligomers are immobile and nucleate VLP growth. A multiplexed acoustic force spectroscopy approach reveals that DNA is compacted in regular steps, suggesting packaging via helical wrapping into a nucleocapsid. By reporting how real-time assembly tracking elucidates viral nucleation and growth principles, our work opens the door to a fundamental understanding of the complex assembly pathways of both VLPs and naturally evolved viruses.

AB - While the structure of a multitude of viral particles has been resolved to atomistic detail, their assembly pathways remain largely elusive. Key unresolved issues are particle nucleation, particle growth, and the mode of genome compaction. These issues are difficult to address in bulk approaches and are effectively only accessible by the real-time tracking of assembly dynamics of individual particles. This we do here by studying the assembly into rod-shaped viruslike particles (VLPs) of artificial capsid polypeptides. Using fluorescence optical tweezers, we establish that small oligomers perform one-dimensional diffusion along the DNA. Larger oligomers are immobile and nucleate VLP growth. A multiplexed acoustic force spectroscopy approach reveals that DNA is compacted in regular steps, suggesting packaging via helical wrapping into a nucleocapsid. By reporting how real-time assembly tracking elucidates viral nucleation and growth principles, our work opens the door to a fundamental understanding of the complex assembly pathways of both VLPs and naturally evolved viruses.

KW - Self-assembly

KW - artificial virus

KW - physical virology

KW - biophysics

KW - optical tweezers

KW - acoustic force spectroscopy

KW - OPTICAL TWEEZERS

KW - DNA

KW - DYNAMICS

KW - PROTEINS

KW - NUCLEATION

KW - COAT

U2 - 10.1021/acs.nanolett.9b02376

DO - 10.1021/acs.nanolett.9b02376

M3 - Article

VL - 19

SP - 5746

EP - 5753

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 8

ER -

ID: 95092499