Publication

Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes

van der Geld, Y. M., Hellmark, T., Selga, D., Heeringa, P., Huitema, M. G., Limburg, P. C. & Kallenberg, C. G. M., Dec-2007, In : Annals of the Rheumatic Diseases. 66, 12, p. 1679-1682 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van der Geld, Y. M., Hellmark, T., Selga, D., Heeringa, P., Huitema, M. G., Limburg, P. C., & Kallenberg, C. G. M. (2007). Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes. Annals of the Rheumatic Diseases, 66(12), 1679-1682. https://doi.org/10.1136/ard.2006.064626

Author

van der Geld, Ymke M. ; Hellmark, Thomas ; Selga, Daina ; Heeringa, Peter ; Huitema, Minke G. ; Limburg, Pieter C. ; Kallenberg, Cees G. M. / Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes. In: Annals of the Rheumatic Diseases. 2007 ; Vol. 66, No. 12. pp. 1679-1682.

Harvard

van der Geld, YM, Hellmark, T, Selga, D, Heeringa, P, Huitema, MG, Limburg, PC & Kallenberg, CGM 2007, 'Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes' Annals of the Rheumatic Diseases, vol. 66, no. 12, pp. 1679-1682. https://doi.org/10.1136/ard.2006.064626

Standard

Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes. / van der Geld, Ymke M.; Hellmark, Thomas; Selga, Daina; Heeringa, Peter; Huitema, Minke G.; Limburg, Pieter C.; Kallenberg, Cees G. M.

In: Annals of the Rheumatic Diseases, Vol. 66, No. 12, 12.2007, p. 1679-1682.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van der Geld YM, Hellmark T, Selga D, Heeringa P, Huitema MG, Limburg PC et al. Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes. Annals of the Rheumatic Diseases. 2007 Dec;66(12):1679-1682. https://doi.org/10.1136/ard.2006.064626


BibTeX

@article{d37937bcb2ae415c85821dd433af7dc3,
title = "Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes",
abstract = "Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice.Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination.Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised with the chimeric human/ mouse PR3 Hmm, HmH and mmH, or a pool of the chimeric human/ mouse PR3 proteins, produced antibodies selectively binding to rat granulocytes as detected by IIF. No gross pathological abnormalities could be detected in kidney or lungs of mice or rats immunised with chimeric human/ mouse PR3.Conclusion: Immunisation with chimeric human/ mouse proteins induces autoantibodies to PR3 in rats and mice. Chimeric proteins can be instrumental in developing experimental models for autoimmune diseases.",
keywords = "ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES, WEGENERS GRANULOMATOSIS, MURINE HOMOLOG, IN-VIVO, VASCULITIS, MYELOPEROXIDASE, GLOMERULONEPHRITIS, NEUTROPHILS, EXPRESSION",
author = "{van der Geld}, {Ymke M.} and Thomas Hellmark and Daina Selga and Peter Heeringa and Huitema, {Minke G.} and Limburg, {Pieter C.} and Kallenberg, {Cees G. M.}",
note = "Article 0",
year = "2007",
month = "12",
doi = "10.1136/ard.2006.064626",
language = "English",
volume = "66",
pages = "1679--1682",
journal = "Annals of the Rheumatic Diseases",
issn = "0003-4967",
publisher = "BMJ PUBLISHING GROUP",
number = "12",

}

RIS

TY - JOUR

T1 - Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes

AU - van der Geld, Ymke M.

AU - Hellmark, Thomas

AU - Selga, Daina

AU - Heeringa, Peter

AU - Huitema, Minke G.

AU - Limburg, Pieter C.

AU - Kallenberg, Cees G. M.

N1 - Article 0

PY - 2007/12

Y1 - 2007/12

N2 - Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice.Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination.Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised with the chimeric human/ mouse PR3 Hmm, HmH and mmH, or a pool of the chimeric human/ mouse PR3 proteins, produced antibodies selectively binding to rat granulocytes as detected by IIF. No gross pathological abnormalities could be detected in kidney or lungs of mice or rats immunised with chimeric human/ mouse PR3.Conclusion: Immunisation with chimeric human/ mouse proteins induces autoantibodies to PR3 in rats and mice. Chimeric proteins can be instrumental in developing experimental models for autoimmune diseases.

AB - Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice.Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination.Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised with the chimeric human/ mouse PR3 Hmm, HmH and mmH, or a pool of the chimeric human/ mouse PR3 proteins, produced antibodies selectively binding to rat granulocytes as detected by IIF. No gross pathological abnormalities could be detected in kidney or lungs of mice or rats immunised with chimeric human/ mouse PR3.Conclusion: Immunisation with chimeric human/ mouse proteins induces autoantibodies to PR3 in rats and mice. Chimeric proteins can be instrumental in developing experimental models for autoimmune diseases.

KW - ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES

KW - WEGENERS GRANULOMATOSIS

KW - MURINE HOMOLOG

KW - IN-VIVO

KW - VASCULITIS

KW - MYELOPEROXIDASE

KW - GLOMERULONEPHRITIS

KW - NEUTROPHILS

KW - EXPRESSION

U2 - 10.1136/ard.2006.064626

DO - 10.1136/ard.2006.064626

M3 - Article

VL - 66

SP - 1679

EP - 1682

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

SN - 0003-4967

IS - 12

ER -

ID: 2024750