Publication

Rat liver slices as a tool to study LPS-induced inflammatory response in the liver

Olinga, P., Merema, M. T., de Jager, MH., Derks, F., Melgert, BN., Moshage, H., Slooff, MJH., Meijer, DKF., Poelstra, K. & Groothuis, GMM., Aug-2001, In : Journal of Hepatology. 35, 2, p. 187-194 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Olinga, P., Merema, M. T., de Jager, MH., Derks, F., Melgert, BN., Moshage, H., ... Groothuis, GMM. (2001). Rat liver slices as a tool to study LPS-induced inflammatory response in the liver. Journal of Hepatology, 35(2), 187-194. https://doi.org/10.1016/S0168-8278(01)00103-9

Author

Olinga, P ; Merema, M.T. ; de Jager, MH ; Derks, F ; Melgert, BN ; Moshage, H ; Slooff, MJH ; Meijer, DKF ; Poelstra, K ; Groothuis, GMM. / Rat liver slices as a tool to study LPS-induced inflammatory response in the liver. In: Journal of Hepatology. 2001 ; Vol. 35, No. 2. pp. 187-194.

Harvard

Olinga, P, Merema, MT, de Jager, MH, Derks, F, Melgert, BN, Moshage, H, Slooff, MJH, Meijer, DKF, Poelstra, K & Groothuis, GMM 2001, 'Rat liver slices as a tool to study LPS-induced inflammatory response in the liver', Journal of Hepatology, vol. 35, no. 2, pp. 187-194. https://doi.org/10.1016/S0168-8278(01)00103-9

Standard

Rat liver slices as a tool to study LPS-induced inflammatory response in the liver. / Olinga, P; Merema, M.T.; de Jager, MH; Derks, F; Melgert, BN; Moshage, H; Slooff, MJH; Meijer, DKF; Poelstra, K; Groothuis, GMM.

In: Journal of Hepatology, Vol. 35, No. 2, 08.2001, p. 187-194.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Olinga P, Merema MT, de Jager MH, Derks F, Melgert BN, Moshage H et al. Rat liver slices as a tool to study LPS-induced inflammatory response in the liver. Journal of Hepatology. 2001 Aug;35(2):187-194. https://doi.org/10.1016/S0168-8278(01)00103-9


BibTeX

@article{5f9fffb1f89e4c4e8656a4c6bef9f3f9,
title = "Rat liver slices as a tool to study LPS-induced inflammatory response in the liver",
abstract = "Background/Aims: Inflammation in the liver is a complex interaction between parenchymal and non-parenchymal cells, and therefore can not be studied in vitro in pure cultures of these cells.Methods: We investigated whether Kupffer cells in the liver slice are still responsive to an inflammatory stimulus of lipopolysaccharide (LPS), and evoke an inflammatory response in the hepatocytes.Results: TNF alpha, IL-1 beta and IL-10 were significantly elevated in culture medium of LPS-stimulated rat liver slices. Nitric oxide (NO) production of LPS-treated slices gradually increased from 5 to 24 h (24 h: 81 +/- 5 muM vs. 14 +/- 2 muM in control P <0.05), paralleled by inducible nitric oxide synthase (iNOS) in the hepatocytes, iNOS mRNA was induced after 3 h. NO production but not iNOS induction was significantly inhibited by NOS inhibitors S-methylisothiourea and N-G-nitro-L-arginine methylester. Both pentoxifylline and dexamethasone inhibited TNF alpha and IL-1 beta production, albeit to a different extent, iNOS induction and, as a result thereof, NO production.Conclusions: These results imply that non-parenchymal cells in liver slices are viable and can be activated by LPS. In addition, it is concluded that the upregulation of iNOS in hepatocytes by LPS is caused by cytokines produced by Kupffer cells because inhibition of TNF alpha and IL-1 beta production attenuated iNOS induction. (C) 2001 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.",
keywords = "cytokines, nitric oxide, iNOS, Kupffer cells, NITRIC-OXIDE SYNTHASE, TUMOR-NECROSIS-FACTOR, CYTOKINE GENE-EXPRESSION, IN-VITRO, KUPFFER CELLS, TISSUE-SLICES, HEPATOCYTES, LIPOPOLYSACCHARIDE, ENDOTOXIN, INTERLEUKIN-1-BETA",
author = "P Olinga and M.T. Merema and {de Jager}, MH and F Derks and BN Melgert and H Moshage and MJH Slooff and DKF Meijer and K Poelstra and GMM Groothuis",
year = "2001",
month = "8",
doi = "10.1016/S0168-8278(01)00103-9",
language = "English",
volume = "35",
pages = "187--194",
journal = "Journal of Hepatology",
issn = "0168-8278",
publisher = "ELSEVIER SCIENCE BV",
number = "2",

}

RIS

TY - JOUR

T1 - Rat liver slices as a tool to study LPS-induced inflammatory response in the liver

AU - Olinga, P

AU - Merema, M.T.

AU - de Jager, MH

AU - Derks, F

AU - Melgert, BN

AU - Moshage, H

AU - Slooff, MJH

AU - Meijer, DKF

AU - Poelstra, K

AU - Groothuis, GMM

PY - 2001/8

Y1 - 2001/8

N2 - Background/Aims: Inflammation in the liver is a complex interaction between parenchymal and non-parenchymal cells, and therefore can not be studied in vitro in pure cultures of these cells.Methods: We investigated whether Kupffer cells in the liver slice are still responsive to an inflammatory stimulus of lipopolysaccharide (LPS), and evoke an inflammatory response in the hepatocytes.Results: TNF alpha, IL-1 beta and IL-10 were significantly elevated in culture medium of LPS-stimulated rat liver slices. Nitric oxide (NO) production of LPS-treated slices gradually increased from 5 to 24 h (24 h: 81 +/- 5 muM vs. 14 +/- 2 muM in control P <0.05), paralleled by inducible nitric oxide synthase (iNOS) in the hepatocytes, iNOS mRNA was induced after 3 h. NO production but not iNOS induction was significantly inhibited by NOS inhibitors S-methylisothiourea and N-G-nitro-L-arginine methylester. Both pentoxifylline and dexamethasone inhibited TNF alpha and IL-1 beta production, albeit to a different extent, iNOS induction and, as a result thereof, NO production.Conclusions: These results imply that non-parenchymal cells in liver slices are viable and can be activated by LPS. In addition, it is concluded that the upregulation of iNOS in hepatocytes by LPS is caused by cytokines produced by Kupffer cells because inhibition of TNF alpha and IL-1 beta production attenuated iNOS induction. (C) 2001 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.

AB - Background/Aims: Inflammation in the liver is a complex interaction between parenchymal and non-parenchymal cells, and therefore can not be studied in vitro in pure cultures of these cells.Methods: We investigated whether Kupffer cells in the liver slice are still responsive to an inflammatory stimulus of lipopolysaccharide (LPS), and evoke an inflammatory response in the hepatocytes.Results: TNF alpha, IL-1 beta and IL-10 were significantly elevated in culture medium of LPS-stimulated rat liver slices. Nitric oxide (NO) production of LPS-treated slices gradually increased from 5 to 24 h (24 h: 81 +/- 5 muM vs. 14 +/- 2 muM in control P <0.05), paralleled by inducible nitric oxide synthase (iNOS) in the hepatocytes, iNOS mRNA was induced after 3 h. NO production but not iNOS induction was significantly inhibited by NOS inhibitors S-methylisothiourea and N-G-nitro-L-arginine methylester. Both pentoxifylline and dexamethasone inhibited TNF alpha and IL-1 beta production, albeit to a different extent, iNOS induction and, as a result thereof, NO production.Conclusions: These results imply that non-parenchymal cells in liver slices are viable and can be activated by LPS. In addition, it is concluded that the upregulation of iNOS in hepatocytes by LPS is caused by cytokines produced by Kupffer cells because inhibition of TNF alpha and IL-1 beta production attenuated iNOS induction. (C) 2001 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.

KW - cytokines

KW - nitric oxide

KW - iNOS

KW - Kupffer cells

KW - NITRIC-OXIDE SYNTHASE

KW - TUMOR-NECROSIS-FACTOR

KW - CYTOKINE GENE-EXPRESSION

KW - IN-VITRO

KW - KUPFFER CELLS

KW - TISSUE-SLICES

KW - HEPATOCYTES

KW - LIPOPOLYSACCHARIDE

KW - ENDOTOXIN

KW - INTERLEUKIN-1-BETA

U2 - 10.1016/S0168-8278(01)00103-9

DO - 10.1016/S0168-8278(01)00103-9

M3 - Article

VL - 35

SP - 187

EP - 194

JO - Journal of Hepatology

JF - Journal of Hepatology

SN - 0168-8278

IS - 2

ER -

ID: 3963767