Publication

Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell-Adhesive

Gehlen, D. B., Novaes, L. C. D. L., Long, W., Ruff, A. J., Jakob, F., Haraszti, T., Chandorkar, Y., Yang, L., van Rijn, P., Schwaneberg, U. & De Laporte, L., 6-Nov-2019, In : ACS Applied Materials & Interfaces. 11, 44, p. 41091-41099 9 p.

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  • Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell Adhesive

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  • Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell-Adhesive

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DOI

  • David B. Gehlen
  • Leticia C. de Lencastre Novaes
  • Wei Long
  • Anna Joelle Ruff
  • Felix Jakob
  • Tamas Haraszti
  • Yashoda Chandorkar
  • Liangliang Yang
  • Patrick van Rijn
  • Ulrich Schwaneberg
  • Laura De Laporte

Polydimethylsiloxane (PDMS) is a synthetic material with excellent properties for biomedical applications because of its easy fabrication method, high flexibility, permeability to oxygen, transparency, and potential to produce high-resolution structures in the case of lithography. However, PDMS needs to be modified to support homogeneous cell attachments and spreading. Even though many physical and chemical methods, like plasma treatment or extracellular matrix coatings, have been developed over the last decades to increase cell surface interactions, these methods are still very time-consuming, often not efficient enough, complex, and can require several treatment steps. To overcome these issues, we present a novel, robust, and fast one-step PDMS coating method using engineered anchor peptides fused to the cell-adhesive peptide sequence (glycine-arginine-glycine-aspartate-serine, GRGDS). The anchor peptide attaches to the PDMS surface predominantly by by simply dipping PDMS in a solution containing the anchor peptide, presenting the GRGDS sequence on the surface available for cell adhesion. The binding performance and kinetics of the anchor peptide to PDMS are characterized, and the coatings are optimized for efficient cell attachment of fibroblasts and endothelial cells. Additionally, the applicability is proven using PDMS-based directional nanotopographic gradients, showing a lower threshold of 5 mu m wrinkles for fibroblast alignment.

Original languageEnglish
Pages (from-to)41091-41099
Number of pages9
JournalACS Applied Materials & Interfaces
Volume11
Issue number44
Early online date2019
Publication statusPublished - 6-Nov-2019

    Keywords

  • polydimethylsiloxane, protein engineering, anchor peptide, liquid chromatography peak I, LCI, RGD, bioactive surface coating, cell adhesion, ON-A-CHIP, SCREENING PLATFORM, POLY(DIMETHYLSILOXANE), PEPTIDE, PROLIFERATION, POLYPROPYLENE, ADSORPTION, INTERPLAY, GUIDANCE, DEVICES

ID: 99605922