Publication

Quantitative on-line monitoring of cellular glucose and lactate metabolism in vitro with slow perfusion

Leegsma-Vogt, G., Venema, K., Brouwer, N., Gramsbergen, JB., Copray, S. & Korf, J., 15-Sep-2004, In : Analytical Chemistry. 76, 18, p. 5431-5435 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

An on-line in vitro perfusion technique is described that allows the continuous quantification of cellular glucose metabolism in vitro. Using biosensor technology, we measure glucose and lactate metabolism at a minute-to-minute time resolution for periods up to several days. The application of our perfusion-detection technique for in vitro monitoring is demonstrated in a wide variety of cells, including primary neuronal and astroglia cultures, yeast cells, and human lymphocytes. The method shows that variations in oxygen delivery or exposure to a noncompetitive pseudosubstrate (here 2-deoxyglucose) affects normal glucose metabolism. The innovative advantage of the present system is that, in contrast to other devices including a recently described system, metabolism per cell can be quantified. The potential of in vitro on-line monitoring is discussed for application in studying normal and abnormal metabolism, toxic and nontoxic drug effects, and human tissue biopsies.

Original languageEnglish
Pages (from-to)5431-5435
Number of pages5
JournalAnalytical Chemistry
Volume76
Issue number18
Publication statusPublished - 15-Sep-2004

    Keywords

  • HOLLOW-FIBER BIOREACTOR, RAT-BRAIN, ULTRASLOW MICRODIALYSIS, HYBRIDOMA CELLS, ENERGY, ULTRAFILTRATION, BIOSENSOR, CULTURE, SPECTROSCOPY, COMBINATION

ID: 4260594